| Molecule Name/ Site Name | Km (uM) | kcat (1/s) | Ratio (k2/k3) | Enzyme Type | Substrate | Product |
1 |
Enzyme Activity: Deph-thr286
Enzyme Molecule: PP1-active | 5.09905 | 2.5 | 4 | explicit E-S complex | CaMKII-thr286*-C aM
| CaMKII-CaM
|
2 |
Enzyme Activity: Deph-thr286b
Enzyme Molecule: PP1-active | 5.09905 | 2.5 | 4 | explicit E-S complex | CaMKII-thr286
| CaMKII
|
3 |
Enzyme Activity: Deph-thr286c
Enzyme Molecule: PP1-active | 5.09905 | 2.5 | 4 | explicit E-S complex | CaMKII***
| CaMK-thr305
|
4 |
Enzyme Activity: Deph-thr305
Enzyme Molecule: PP1-active | 5.09905 | 2.5 | 4 | explicit E-S complex | CaMKII***
| CaMKII-thr286
|
5 |
Enzyme Activity: Deph-thr305a
Enzyme Molecule: PP1-active | 5.09905 | 2.5 | 4 | explicit E-S complex | CaMK-thr305
| CaMKII
|
6 |
Enzyme Activity: phosph-AC2
Enzyme Molecule: PKC-active | 33.3337 | 4 | 4 | explicit E-S complex | AC2
| AC2*
|
| Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4 |
7 |
Enzyme Activity: PKC-phosph-neuro granin
Enzyme Molecule: PKC-active | 28.6273 | 0.58 | 4.03448 | explicit E-S complex | neurogranin
| neurogranin*
|
| Rates from Huang et al ABB 305:2 570-580 1993 |
8 |
Enzyme Activity:
PKC-phosph-neuro granin_ PSD
Enzyme Molecule: PKC-active | 28.6273 | 0.58 | 4.03448 | explicit E-S complex | neurogranin_PSD
| neurogranin*_ PSD
|
| Rates from Huang et al ABB 305:2 570-580 1993 |
9 |
Enzyme Activity: PKC-phosph-ng-Ca M
Enzyme Molecule: PKC-active | 28.5956 | 0.35 | 4 | explicit E-S complex | neurogranin-CaM
| CaM neurogranin*
|
| Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 |
10 |
Enzyme Activity:
PKC-phosph-ng-Ca M_ PSD
Enzyme Molecule: PKC-active | 28.5956 | 0.35 | 4 | explicit E-S complex | neurogranin-CaM_ PSD
| CaM-PSD neurogranin*_ PSD
|
| Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 |
11 |
Enzyme Activity: PP2A-dephosph-I1
Enzyme Molecule: PP2A | 15.9999 | 2 | 4.1667 | explicit E-S complex | I1*
| I1
|
| PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6 |
12 |
Enzyme Activity:
PP2A-dephosph-I1 _ PSD
Enzyme Molecule: PP2A | 15.9999 | 2 | 4.1667 | explicit E-S complex | I1*
| I1
|
| PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6 |
13 |
Enzyme Activity: PP2A-dephosph-PP 1-I*
Enzyme Molecule: PP2A | 15.9999 | 2 | 4.1667 | explicit E-S complex | PP1-I1*
| PP1-I1
|
| k1 changed from 3.3e-6 to 6.6e-6 |
14 |
Enzyme Activity:
PP2A-dephosph-PP 1-I*_ PSD
Enzyme Molecule: PP2A | 15.9999 | 2 | 4.1667 | explicit E-S complex | PP1-I1*
| PP1-I1
|
| k1 changed from 3.3e-6 to 6.6e-6 |