This is a model of weak coupling between the AMPAR traffikcing bistability, and the CaMKII autophosphorylation bistability. In this model, there are three stable states: Both off, AMPAR on, or both on. The fourth possible state: CaMKII on but AMPAR off, is not truly stable, since over the course of hours the AMPAR also turns on. Thu Aug 9 17:15:40 2007 network Upinder S. Bhalla, NCBS Arnold Hayer and Upinder S. Bhalla, NCBS General Mammalian Neuronal Synaptic Spine, Postsynaptic Density Quantitative match to experiments, Qualitative Exact GENESIS implementation Replicates original data , Approximates original data ATP is present in all cells between 2 and 10 mM. See Lehninger This isn\'t explicitly present in the M&L model, but is obviously needed. I assume its conc is fixed at 1uM for now, which is a bit high. PLA2 is stim 7x by PIP2 @ 0.5 uM (Leslie and Channon BBA 1045:261(1990) Leslie and Channon say PIP2 is present at 0.1 - 0.2mol% range in membs, which comes to 50 nM. Ref is Majerus et al Cell 37 pp 701-703 1984 Lets use a lower level of 30 nM, same ref.... $\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}+\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286\_star\_\_minus\_CaM}$ $\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}+\mathrm{CaMKII\_slash\_CaMKII\_star\_\_star\_\_star\_}+\mathrm{CaMKII\_slash\_basal\_CaMKII\_cyt}$ $\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}+\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286\_star\_\_minus\_CaM}+\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}+\mathrm{CaMKII\_slash\_CaMKII\_star\_\_star\_\_star\_}+\mathrm{CaMKII\_slash\_CaMK\_minus\_thr305}+\mathrm{CaMKII\_slash\_CaMKII}+\mathrm{CaMKII\_slash\_basal\_CaMKII\_cyt}$ $\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII}+\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII}$ $\mathrm{AMPAR\_slash\_GluR23\_M}+\mathrm{tot\_mem\_GluR12}$ $\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B}+\mathrm{AMPAR\_slash\_A845\_star\_\_B}+\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_slash\_A\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_slash\_A831\_star\_\_B845\_star\_}+\mathrm{AMPAR\_slash\_A\_B845\_star\_}$ $\mathrm{I\_845}+\mathrm{I\_845\_minus\_P}+\mathrm{I\_845\_PP}$ $\mathrm{tot\_I\_GluR12}+\mathrm{AMPAR\_slash\_GluR23\_I}$ $\mathrm{AMPAR\_memb\_slash\_A\_B}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}$ $\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}$ $\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}$ $\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A\_B}$ $\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}+\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}$ $\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}+\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}$ $\mathrm{Ser845}+\mathrm{Ser845\_minus\_P}+\mathrm{Ser845\_minus\_PP}$ $\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_}+\mathrm{AMPAR\_slash\_A\_B831\_star\_}+\mathrm{AMPAR\_slash\_A831\_star\_\_B}+\mathrm{AMPAR\_slash\_A\_B}$ $\mathrm{AMPAR\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}+\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_845\_star\_}+\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B845\_star\_}+\mathrm{AMPAR\_slash\_A845\_star\_\_B845\_star\_}$ $\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}$ $\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_star\_\_star\_\_star\_\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_basal\_CaMKII\_PSD}$ $\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr305\_minus\_PSD}$ $\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD}$ $\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}+\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}$ This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol. kf*CaM_minus_Ca4*CaMKII_slash_CaMKII-kb*CaMKII_slash_CaMKII_minus_CaM $\mathrm{kf}\mathrm{CaM\_minus\_Ca4}\mathrm{CaMKII\_slash\_CaMKII}-\mathrm{kb}\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}$ Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 kf*CaMKII_slash_CaMKII_minus_thr286*CaM_minus_Ca4-kb*CaMKII_slash_CaMKII_minus_thr286_star__minus_CaM $\mathrm{kf}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}\mathrm{CaM\_minus\_Ca4}-\mathrm{kb}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286\_star\_\_minus\_CaM}$ Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !).... kf*CaM_slash_CaM*Ca^2-kb*CaM_minus_TR2_minus_Ca2 $\mathrm{kf}\mathrm{CaM\_slash\_CaM}\mathrm{Ca}^2-\mathrm{kb}\mathrm{CaM\_minus\_TR2\_minus\_Ca2}$ K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10 kf*CaM_minus_TR2_minus_Ca2*Ca-kb*CaM_minus_Ca3 $\mathrm{kf}\mathrm{CaM\_minus\_TR2\_minus\_Ca2}\mathrm{Ca}-\mathrm{kb}\mathrm{CaM\_minus\_Ca3}$ Surprisingly, no direct info on rates from neurogranin at this time. These rates are based on GAP-43 binding studies. As GAP-43 and neurogranin share near identity in the CaM/PKC binding regions, and also similarity in phosph and dephosph rates, I am borrowing GAP-43 kinetic info. See Alexander et al JBC 262:13 6108-6113 1987 kf*CaM_slash_neurogranin*CaM_slash_CaM-kb*CaM_slash_neurogranin_minus_CaM $\mathrm{kf}\mathrm{CaM\_slash\_neurogranin}\mathrm{CaM\_slash\_CaM}-\mathrm{kb}\mathrm{CaM\_slash\_neurogranin\_minus\_CaM}$ This is put in to keep the basal levels of neurogranin* experimentally reasonable. From various papers, specially Ramakers et al JBC 270:23 1995 13892-13898, it looks like the basal level of phosph is between 20 and 40%. I est around 25 % The kf of 0.005 gives around this level at basal PKC activity levels of 0.1 uM active PKC. kf*CaM_slash_neurogranin_star_-kb*CaM_slash_neurogranin $\mathrm{kf}\mathrm{CaM\_slash\_neurogranin\_star\_}-\mathrm{kb}\mathrm{CaM\_slash\_neurogranin}$ Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !).... kf*CaM_slash_CaM_minus_PSD*Ca_minus_PSD^2-kb*CaM_minus_TR2_minus_Ca2_minus_PSD $\mathrm{kf}\mathrm{CaM\_slash\_CaM\_minus\_PSD}\mathrm{Ca\_minus\_PSD}^2-\mathrm{kb}\mathrm{CaM\_minus\_TR2\_minus\_Ca2\_minus\_PSD}$ K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10 kf*CaM_minus_TR2_minus_Ca2_minus_PSD*Ca_minus_PSD-kb*CaM_minus_Ca3_minus_PSD $\mathrm{kf}\mathrm{CaM\_minus\_TR2\_minus\_Ca2\_minus\_PSD}\mathrm{Ca\_minus\_PSD}-\mathrm{kb}\mathrm{CaM\_minus\_Ca3\_minus\_PSD}$ Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10 kf*CaM_minus_Ca3_minus_PSD*Ca_minus_PSD-kb*CaM_minus_Ca4_minus_PSD $\mathrm{kf}\mathrm{CaM\_minus\_Ca3\_minus\_PSD}\mathrm{Ca\_minus\_PSD}-\mathrm{kb}\mathrm{CaM\_minus\_Ca4\_minus\_PSD}$ Surprisingly, no direct info on rates from neurogranin at this time. These rates are based on GAP-43 binding studies. As GAP-43 and neurogranin share near identity in the CaM/PKC binding regions, and also similarity in phosph and dephosph rates, I am borrowing GAP-43 kinetic info. See Alexander et al JBC 262:13 6108-6113 1987 kf*CaM_slash_neurogranin_PSD*CaM_slash_CaM_minus_PSD-kb*CaM_slash_neurogranin_minus_CaM_PSD $\mathrm{kf}\mathrm{CaM\_slash\_neurogranin\_PSD}\mathrm{CaM\_slash\_CaM\_minus\_PSD}-\mathrm{kb}\mathrm{CaM\_slash\_neurogranin\_minus\_CaM\_PSD}$ This is put in to keep the basal levels of neurogranin* experimentally reasonable. From various papers, specially Ramakers et al JBC 270:23 1995 13892-13898, it looks like the basal level of phosph is between 20 and 40%. I est around 25 % The kf of 0.005 gives around this level at basal PKC activity levels of 0.1 uM active PKC. kf*CaM_slash_neurogranin_star__PSD-kb*CaM_slash_neurogranin_PSD $\mathrm{kf}\mathrm{CaM\_slash\_neurogranin\_star\_\_PSD}-\mathrm{kb}\mathrm{CaM\_slash\_neurogranin\_PSD}$ K inhib = 1nM from Cohen Ann Rev Bioch 1989, 4 nM from Foukes et al Assume 2 nM. kf /kb = 8.333e-4 kf*PP1_slash_I1_star_*PP1_minus_active-kb*PP1_slash_PP1_minus_I1_star_ $\mathrm{kf}\mathrm{PP1\_slash\_I1\_star\_}\mathrm{PP1\_minus\_active}-\mathrm{kb}\mathrm{PP1\_slash\_PP1\_minus\_I1\_star\_}$ Let us assume that the equil in this case is very far over to the right. This is probably safe. kf*PP1_slash_PP1_minus_I1-kb*PP1_minus_active*PP1_slash_I1 $\mathrm{kf}\mathrm{PP1\_slash\_PP1\_minus\_I1}-\mathrm{kb}\mathrm{PP1\_minus\_active}\mathrm{PP1\_slash\_I1}$ This process is probably much more complicated and involves CaM. However, as I can\'t find detailed info I am bundling this into a single step. Based on Steemer and Klee pg 6863, the Kact is 0.5 uM. kf/kb = 1/(0.5 * 6e5)^2 = 1.11e-11 kf*Ca^2*PP2B_slash_CaNAB_minus_Ca2-kb*PP2B_slash_CaNAB_minus_Ca4 $\mathrm{kf}\mathrm{Ca}^2\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca2}-\mathrm{kb}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4}$ going on the experience with CaM, we put the fast (high affinity) sites first. We only know (Stemmer and Klee) that the affinity is < 70 nM. Assuming 10 nM at first, we get kf = 2.78e-8, kb = 1. Try 20 nM. kf = 7e-9, kb = 1 kf*PP2B_slash_CaNAB*Ca^2-kb*PP2B_slash_CaNAB_minus_Ca2 $\mathrm{kf}\mathrm{PP2B\_slash\_CaNAB}\mathrm{Ca}^2-\mathrm{kb}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca2}$ kf*CaM_minus_Ca4*PP2B_slash_CaNAB_minus_Ca4-kb*PP2B_slash_CaM_Ca_n_minus_CaNAB $\mathrm{kf}\mathrm{CaM\_minus\_Ca4}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4}-\mathrm{kb}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}$ Hasler et al FASEB J 6:2734-2741 1992 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al PNAS USA 78:3 1591-1595 1981 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell). Ogreid and Doskeland Febs Lett 129:2 287-292 1981 have figs suggesting time course of complete assoc is < 1 min. kf*PKA_slash_R2C2*cAMP-kb*PKA_slash_R2C2_minus_cAMP $\mathrm{kf}\mathrm{PKA\_slash\_R2C2}\mathrm{cAMP}-\mathrm{kb}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP}$ For now let us set this to the same Km (1e-7M) as site B. This gives kf/kb = .7e-7M * 1e6 / (6e5^2) : 1/(6e5^2) = 2e-13:2.77e-12 Smith et al have better values. They say that this is cooperative, so the consts are now kf/kb =8.3e-4 kf*PKA_slash_R2C2_minus_cAMP*cAMP-kb*PKA_slash_R2C2_minus_cAMP2 $\mathrm{kf}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP}\mathrm{cAMP}-\mathrm{kb}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP2}$ kf*PKA_slash_R2C2_minus_cAMP2*cAMP-kb*PKA_slash_R2C2_minus_cAMP3 $\mathrm{kf}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP2}\mathrm{cAMP}-\mathrm{kb}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP3}$ kf*cAMP*PKA_slash_R2C2_minus_cAMP3-kb*PKA_slash_R2C2_minus_cAMP4 $\mathrm{kf}\mathrm{cAMP}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP3}-\mathrm{kb}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP4}$ This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10 kf*PKA_slash_R2C2_minus_cAMP4-kb*PKA_slash_PKA_minus_active*PKA_slash_R2C_minus_cAMP4 $\mathrm{kf}\mathrm{PKA\_slash\_R2C2\_minus\_cAMP4}-\mathrm{kb}\mathrm{PKA\_slash\_PKA\_minus\_active}\mathrm{PKA\_slash\_R2C\_minus\_cAMP4}$ kf*PKA_slash_R2C_minus_cAMP4-kb*PKA_slash_PKA_minus_active*PKA_slash_R2_minus_cAMP4 $\mathrm{kf}\mathrm{PKA\_slash\_R2C\_minus\_cAMP4}-\mathrm{kb}\mathrm{PKA\_slash\_PKA\_minus\_active}\mathrm{PKA\_slash\_R2\_minus\_cAMP4}$ This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though. kf = 1e-5 kb = 1 kf*PKA_slash_PKA_minus_active*PKA_slash_PKA_minus_inhibitor-kb*PKA_slash_inhibited_minus_PKA $\mathrm{kf}\mathrm{PKA\_slash\_PKA\_minus\_active}\mathrm{PKA\_slash\_PKA\_minus\_inhibitor}-\mathrm{kb}\mathrm{PKA\_slash\_inhibited\_minus\_PKA}$ Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5 kf*CaM_minus_Ca4*AC_slash_AC1-kb*AC_slash_AC1_minus_CaM $\mathrm{kf}\mathrm{CaM\_minus\_Ca4}\mathrm{AC\_slash\_AC1}-\mathrm{kb}\mathrm{AC\_slash\_AC1\_minus\_CaM}$ Random rate. kf*AC_slash_AC2_star_-kb*AC_slash_AC2 $\mathrm{kf}\mathrm{AC\_slash\_AC2\_star\_}-\mathrm{kb}\mathrm{AC\_slash\_AC2}$ The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown. kf*AC_slash_cAMP_minus_PDE_star_-kb*AC_slash_cAMP_minus_PDE $\mathrm{kf}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_star\_}-\mathrm{kb}\mathrm{AC\_slash\_cAMP\_minus\_PDE}$ For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3 kf*AC_slash_PDE1*CaM_minus_Ca4-kb*AC_slash_CaM_dot_PDE1 $\mathrm{kf}\mathrm{AC\_slash\_PDE1}\mathrm{CaM\_minus\_Ca4}-\mathrm{kb}\mathrm{AC\_slash\_CaM\_dot\_PDE1}$ Represents diffusion, from a volume of 9e-20 to 5e-18. Assuming neck dimensions of 0.1 x 0.1 microns, this works out to a diffusion const of about 270 um^2/sec, which is pretty conservative. It is what cAMP does in frog cilia. kf*cAMP-kb*AC_slash_cAMP_in_dend $\mathrm{kf}\mathrm{cAMP}-\mathrm{kb}\mathrm{AC\_slash\_cAMP\_in\_dend}$ Diffusional equilibrium between PSD- and cytosolic compartment. According to D. Bary in Cell Movements 2nd ed 2001 D for proteins is 5e-7 cm^2/s giving 10 ms for a translocation of 1 um. kf*CaM_minus_Ca4_minus_PSD-kb*CaM_minus_Ca4 $\mathrm{kf}\mathrm{CaM\_minus\_Ca4\_minus\_PSD}-\mathrm{kb}\mathrm{CaM\_minus\_Ca4}$ Let us assume that the equil in this case is very far over to the right. This is probably safe. kf*PP1_PSD_slash_PP1_minus_I1-kb*PP1_PSD_slash_I1*PP1_PSD_slash_PP1_minus_active_PSD $\mathrm{kf}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_I1}-\mathrm{kb}\mathrm{PP1\_PSD\_slash\_I1}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}$ K inhib = 1nM from Cohen Ann Rev Bioch 1989, 4 nM from Foukes et al Assume 2 nM. kf /kb = 8.333e-4 kf*PP1_PSD_slash_I1_star_*PP1_PSD_slash_PP1_minus_active_PSD-kb*PP1_PSD_slash_PP1_minus_I1_star_ $\mathrm{kf}\mathrm{PP1\_PSD\_slash\_I1\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{kb}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_I1\_star\_}$ Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10 kf*CaM_minus_Ca3*Ca-kb*CaM_minus_Ca4 $\mathrm{kf}\mathrm{CaM\_minus\_Ca3}\mathrm{Ca}-\mathrm{kb}\mathrm{CaM\_minus\_Ca4}$ kf*AMPAR_slash_GluR23_M-kb*AMPAR_slash_GluR23_I $\mathrm{kf}\mathrm{AMPAR\_slash\_GluR23\_M}-\mathrm{kb}\mathrm{AMPAR\_slash\_GluR23\_I}$ kf*AMPAR_memb_slash_A831_star__B831_star_-kb*AMPAR_slash_A831_star__B831_star_*Anchor $\mathrm{kf}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_}\mathrm{Anchor}$ kf*AMPAR_memb_slash_A_B831_star_-kb*AMPAR_slash_A_B831_star_*Anchor $\mathrm{kf}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_A\_B831\_star\_}\mathrm{Anchor}$ kf*AMPAR_memb_slash_A831_star__B-kb*AMPAR_slash_A831_star__B*Anchor $\mathrm{kf}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}-\mathrm{kb}\mathrm{AMPAR\_slash\_A831\_star\_\_B}\mathrm{Anchor}$ kf*AMPAR_memb_slash_A_B-kb*AMPAR_slash_A_B*Anchor $\mathrm{kf}\mathrm{AMPAR\_memb\_slash\_A\_B}-\mathrm{kb}\mathrm{AMPAR\_slash\_A\_B}\mathrm{Anchor}$ kf*AMPAR_slash_A835_star_845_star__B835_star_845_star_-kb*AMPAR_slash_AMPAR_deg $\mathrm{kf}\mathrm{AMPAR\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_AMPAR\_deg}$ kf*AMPAR_slash_A845_star__B831_star_845_star_-kb*AMPAR_slash_AMPAR_deg $\mathrm{kf}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_845\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_AMPAR\_deg}$ kf*AMPAR_slash_A831_star_845_star__B845_star_-kb*AMPAR_slash_AMPAR_deg $\mathrm{kf}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B845\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_AMPAR\_deg}$ kf*AMPAR_slash_A845_star__B845_star_-kb*AMPAR_slash_AMPAR_deg $\mathrm{kf}\mathrm{AMPAR\_slash\_A845\_star\_\_B845\_star\_}-\mathrm{kb}\mathrm{AMPAR\_slash\_AMPAR\_deg}$ kf*AMPAR_slash_A835_star_845_star__B835_star_845_star_*Anchor-kb*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_ $\mathrm{kf}\mathrm{AMPAR\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{Anchor}-\mathrm{kb}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}$ kf*AMPAR_slash_A845_star__B831_star_845_star_*Anchor-kb*AMPAR_memb_slash_A845_star__B831_star_845_star_ $\mathrm{kf}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{Anchor}-\mathrm{kb}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}$ kf*AMPAR_slash_A831_star_845_star__B845_star_*Anchor-kb*AMPAR_memb_slash_A831_star_845_star__B845_star_ $\mathrm{kf}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{Anchor}-\mathrm{kb}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}$ Exo rates are scaled from earlier model versions to account for binding of anchor. We have about 200 molecules of anchor in the whole model. Here we select rates to give us a max of about 150 molecules of GluR at the synapse. kf*AMPAR_slash_A845_star__B845_star_*Anchor-kb*AMPAR_memb_slash_A845_star__B845_star_ $\mathrm{kf}\mathrm{AMPAR\_slash\_A845\_star\_\_B845\_star\_}\mathrm{Anchor}-\mathrm{kb}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}$ kf*PKC_minus_control-kb*PKC_minus_active $\mathrm{kf}\mathrm{PKC\_minus\_control}-\mathrm{kb}\mathrm{PKC\_minus\_active}$ kf*Ca_control_cyt-kb*Ca $\mathrm{kf}\mathrm{Ca\_control\_cyt}-\mathrm{kb}\mathrm{Ca}$ kf*Ca_control_PSD-kb*Ca_minus_PSD $\mathrm{kf}\mathrm{Ca\_control\_PSD}-\mathrm{kb}\mathrm{Ca\_minus\_PSD}$ Represents both synthesis and degradation of the receptor. The rate is set to be rather fast for now. The forward rate also includes scaling terms because the AMPAR_bulk is in the dendritic volume of 5e-18. This means that we need to lower Kf to account for the difference in volumes. Effectively Kf is 1/sec, but the scaled version becomes 9e-20/5e-18 = 0.018 kf*AMPAR_bulk-kb*AMPAR_slash_A_B $\mathrm{kf}\mathrm{AMPAR\_bulk}-\mathrm{kb}\mathrm{AMPAR\_slash\_A\_B}$ This reaction comes into play when stochastic calculations are used. If we do not have a stochastic step here then the levels of basal_CaMKII_PSD are just a fixed number, which is probably not a good representation of stochasticity. Having a reaction for the basal activity ensures that the basal activity too exhibits some fluctuations. kf*CaMKII_PSD_slash_basal_CaMKII_PSD_control-kb*CaMKII_PSD_slash_basal_CaMKII_PSD $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_basal\_CaMKII\_PSD\_control}-\mathrm{kb}\mathrm{CaMKII\_PSD\_slash\_basal\_CaMKII\_PSD}$ kf*CaMKII_PSD_slash_CaMKII_minus_CaM_minus_PSD-kb*CaM_minus_Ca4_minus_PSD*CaMKII_PSD_slash_CaMKII_minus_PSD $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}-\mathrm{kb}\mathrm{CaM\_minus\_Ca4\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}$ kf*CaMKII_PSD_slash_CaMKII_minus_PSD*CaM_minus_Ca4_minus_PSD-kb*CaMKII_PSD_slash_CaMKII_minus_CaM_minus_PSD $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}\mathrm{CaM\_minus\_Ca4\_minus\_PSD}-\mathrm{kb}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}$ Same values as for the main compartment Can the main compartment pool of Ca/CaM be used? kf*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_PSD*CaM_minus_Ca4_minus_PSD-kb*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_CaM_minus_PSD $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}\mathrm{CaM\_minus\_Ca4\_minus\_PSD}-\mathrm{kb}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}$ Same as for back_1 kf*CaMKII_PSD_slash_CaMKII_minus_thr305_minus_PSD-kb*CaMKII_slash_CaMK_minus_thr305*CaMKII_PSD_slash_NMDAR $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr305\_minus\_PSD}-\mathrm{kb}\mathrm{CaMKII\_slash\_CaMK\_minus\_thr305}\mathrm{CaMKII\_PSD\_slash\_NMDAR}$ Rates set by the translocation experiments of Shen and Meyer, Science 1999. Note that this reaction also incorporates a translocation between compartments of different volumes. kf*CaMKII_PSD_slash_CaMKII_minus_PSD-kb*CaMKII_slash_CaMKII*CaMKII_PSD_slash_NMDAR $\mathrm{kf}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}-\mathrm{kb}\mathrm{CaMKII\_slash\_CaMKII}\mathrm{CaMKII\_PSD\_slash\_NMDAR}$ Rates to match curve in fig2 from Shen and Meyer, Science 284:162-166(1999), calculated for 6:1 alpha:beta CaMKII heterodimers Note that this reaction also incorporates a translocation between compartments of different volumes. kf*CaMKII_slash_CaMKII_minus_CaM*CaMKII_PSD_slash_NMDAR-kb*CaMKII_PSD_slash_CaMKII_minus_CaM_minus_PSD $\mathrm{kf}\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}\mathrm{CaMKII\_PSD\_slash\_NMDAR}-\mathrm{kb}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}$ Same as for transloc_1 kf*CaMKII_slash_CaMKII_minus_thr286_star__minus_CaM*CaMKII_PSD_slash_NMDAR-kb*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_CaM_minus_PSD $\mathrm{kf}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286\_star\_\_minus\_CaM}\mathrm{CaMKII\_PSD\_slash\_NMDAR}-\mathrm{kb}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}$ k1*CaMKII_slash_CaMKII_minus_thr286_star__minus_CaM*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286\_star\_\_minus\_CaM}\mathrm{PP1\_minus\_active}-\mathrm{k2}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286\_slash\_Deph\_minus\_thr286\_cplx}$ k3*PP1_minus_active_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx $\mathrm{k3}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286\_slash\_Deph\_minus\_thr286\_cplx}$ k1*CaMKII_slash_CaMKII_minus_thr286*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr286b_slash_Deph_minus_thr286b_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}\mathrm{PP1\_minus\_active}-\mathrm{k2}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286b\_slash\_Deph\_minus\_thr286b\_cplx}$ k3*PP1_minus_active_slash_Deph_minus_thr286b_slash_Deph_minus_thr286b_cplx $\mathrm{k3}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286b\_slash\_Deph\_minus\_thr286b\_cplx}$ k1*CaMKII_slash_CaMKII_star__star__star_*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_star\_\_star\_\_star\_}\mathrm{PP1\_minus\_active}-\mathrm{k2}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr305\_slash\_Deph\_minus\_thr305\_cplx}$ k3*PP1_minus_active_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx $\mathrm{k3}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr305\_slash\_Deph\_minus\_thr305\_cplx}$ k1*CaMKII_slash_CaMKII_star__star__star_*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_star\_\_star\_\_star\_}\mathrm{PP1\_minus\_active}-\mathrm{k2}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286c\_slash\_Deph\_minus\_thr286c\_cplx}$ k3*PP1_minus_active_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx $\mathrm{k3}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr286c\_slash\_Deph\_minus\_thr286c\_cplx}$ k1*CaMKII_slash_CaMK_minus_thr305*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr305a_slash_Deph_minus_thr305a_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMK\_minus\_thr305}\mathrm{PP1\_minus\_active}-\mathrm{k2}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr305a\_slash\_Deph\_minus\_thr305a\_cplx}$ k3*PP1_minus_active_slash_Deph_minus_thr305a_slash_Deph_minus_thr305a_cplx $\mathrm{k3}\mathrm{PP1\_minus\_active\_slash\_Deph\_minus\_thr305a\_slash\_Deph\_minus\_thr305a\_cplx}$ Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5. k1*CaMKII_slash_CaMKII_minus_thr286*CaMKII_slash_tot_CaM_CaMKII-k2*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_305_slash_CaM_act_305_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII}-\mathrm{k2}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII\_slash\_CaM\_act\_305\_slash\_CaM\_act\_305\_cplx}$ k3*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_305_slash_CaM_act_305_cplx $\mathrm{k3}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII\_slash\_CaM\_act\_305\_slash\_CaM\_act\_305\_cplx}$ k1*CaMKII_slash_CaMKII_minus_CaM*CaMKII_slash_tot_CaM_CaMKII-k2*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_286_slash_CaM_act_286_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII}-\mathrm{k2}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII\_slash\_CaM\_act\_286\_slash\_CaM\_act\_286\_cplx}$ k3*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_286_slash_CaM_act_286_cplx $\mathrm{k3}\mathrm{CaMKII\_slash\_tot\_CaM\_CaMKII\_slash\_CaM\_act\_286\_slash\_CaM\_act\_286\_cplx}$ See Hanson and Schulman again, for afterburst rates of phosph. k1*CaMKII_slash_CaMKII_minus_thr286*CaMKII_slash_tot_autonomous_CaMKII-k2*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_305_slash_auton_305_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_thr286}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII}-\mathrm{k2}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII\_slash\_auton\_305\_slash\_auton\_305\_cplx}$ k3*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_305_slash_auton_305_cplx $\mathrm{k3}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII\_slash\_auton\_305\_slash\_auton\_305\_cplx}$ k1*CaMKII_slash_CaMKII_minus_CaM*CaMKII_slash_tot_autonomous_CaMKII-k2*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_286_slash_auton_286_cplx $\mathrm{k1}\mathrm{CaMKII\_slash\_CaMKII\_minus\_CaM}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII}-\mathrm{k2}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII\_slash\_auton\_286\_slash\_auton\_286\_cplx}$ k3*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_286_slash_auton_286_cplx $\mathrm{k3}\mathrm{CaMKII\_slash\_tot\_autonomous\_CaMKII\_slash\_auton\_286\_slash\_auton\_286\_cplx}$ PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6 k1*PP1_slash_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_I1_slash_PP2A_minus_dephosph_minus_I1_cplx $\mathrm{k1}\mathrm{PP1\_slash\_I1\_star\_}\mathrm{PP2A}-\mathrm{k2}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_cplx}$ k3*PP2A_slash_PP2A_minus_dephosph_minus_I1_slash_PP2A_minus_dephosph_minus_I1_cplx $\mathrm{k3}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_cplx}$ k1 changed from 3.3e-6 to 6.6e-6 k1*PP1_slash_PP1_minus_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__cplx $\mathrm{k1}\mathrm{PP1\_slash\_PP1\_minus\_I1\_star\_}\mathrm{PP2A}-\mathrm{k2}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_cplx}$ k3*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__cplx $\mathrm{k3}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_cplx}$ PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6 k1*PP1_PSD_slash_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_I1_PSD_slash_PP2A_minus_dephosph_minus_I1_PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_I1\_star\_}\mathrm{PP2A}-\mathrm{k2}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_PSD\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_PSD\_cplx}$ k3*PP2A_slash_PP2A_minus_dephosph_minus_I1_PSD_slash_PP2A_minus_dephosph_minus_I1_PSD_cplx $\mathrm{k3}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_PSD\_slash\_PP2A\_minus\_dephosph\_minus\_I1\_PSD\_cplx}$ k1 changed from 3.3e-6 to 6.6e-6 k1*PP1_PSD_slash_PP1_minus_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__PSD_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_I1\_star\_}\mathrm{PP2A}-\mathrm{k2}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_cplx}$ k3*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__PSD_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__PSD_cplx $\mathrm{k3}\mathrm{PP2A\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_slash\_PP2A\_minus\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_cplx}$ From Seki et al ABB 316(2):673-679 k1*CaM_slash_neurogranin_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_neurogranin_slash_dephosph_neurogranin_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_neurogranin\_slash\_dephosph\_neurogranin\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_neurogranin_slash_dephosph_neurogranin_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_neurogranin\_slash\_dephosph\_neurogranin\_cplx}$ k1*PP1_slash_I1_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_inhib1_slash_dephosph_inhib1_cplx $\mathrm{k1}\mathrm{PP1\_slash\_I1\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_inhib1\_slash\_dephosph\_inhib1\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_inhib1_slash_dephosph_inhib1_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_inhib1\_slash\_dephosph\_inhib1\_cplx}$ k1*PP1_slash_PP1_minus_I1_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__slash_dephosph_minus_PP1_minus_I_star__cplx $\mathrm{k1}\mathrm{PP1\_slash\_PP1\_minus\_I1\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__slash_dephosph_minus_PP1_minus_I_star__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_cplx}$ k1*PP1_PSD_slash_I1_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_inhib1_PSD_slash_dephosph_inhib1_PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_I1\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_inhib1\_PSD\_slash\_dephosph\_inhib1\_PSD\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_inhib1_PSD_slash_dephosph_inhib1_PSD_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_inhib1\_PSD\_slash\_dephosph\_inhib1\_PSD\_cplx}$ k1*PP1_PSD_slash_PP1_minus_I1_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__PSD_slash_dephosph_minus_PP1_minus_I_star__PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_I1\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__PSD_slash_dephosph_minus_PP1_minus_I_star__PSD_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_slash\_dephosph\_minus\_PP1\_minus\_I\_star\_\_PSD\_cplx}$ k1*AMPAR_slash_A845_star__B*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_17_sbc__slash_PP2B_sbo_17_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_17\_sbc\_\_slash\_PP2B\_sbo\_17\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_17_sbc__slash_PP2B_sbo_17_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_17\_sbc\_\_slash\_PP2B\_sbo\_17\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_18_sbc__slash_PP2B_sbo_18_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_18\_sbc\_\_slash\_PP2B\_sbo\_18\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_18_sbc__slash_PP2B_sbo_18_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_18\_sbc\_\_slash\_PP2B\_sbo\_18\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B831_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_19_sbc__slash_PP2B_sbo_19_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_19\_sbc\_\_slash\_PP2B\_sbo\_19\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_19_sbc__slash_PP2B_sbo_19_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_19\_sbc\_\_slash\_PP2B\_sbo\_19\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B831_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_20_sbc__slash_PP2B_sbo_20_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_20\_sbc\_\_slash\_PP2B\_sbo\_20\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_20_sbc__slash_PP2B_sbo_20_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_20\_sbc\_\_slash\_PP2B\_sbo\_20\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_21_sbc__slash_PP2B_sbo_21_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_21\_sbc\_\_slash\_PP2B\_sbo\_21\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_21_sbc__slash_PP2B_sbo_21_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_21\_sbc\_\_slash\_PP2B\_sbo\_21\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_22_sbc__slash_PP2B_sbo_22_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_22\_sbc\_\_slash\_PP2B\_sbo\_22\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_22_sbc__slash_PP2B_sbo_22_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_22\_sbc\_\_slash\_PP2B\_sbo\_22\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_23_sbc__slash_PP2B_sbo_23_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_23\_sbc\_\_slash\_PP2B\_sbo\_23\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_23_sbc__slash_PP2B_sbo_23_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_23\_sbc\_\_slash\_PP2B\_sbo\_23\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_24_sbc__slash_PP2B_sbo_24_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_24\_sbc\_\_slash\_PP2B\_sbo\_24\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_24_sbc__slash_PP2B_sbo_24_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_24\_sbc\_\_slash\_PP2B\_sbo\_24\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_25_sbc__slash_PP2B_sbo_25_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_25\_sbc\_\_slash\_PP2B\_sbo\_25\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_25_sbc__slash_PP2B_sbo_25_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_25\_sbc\_\_slash\_PP2B\_sbo\_25\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_26_sbc__slash_PP2B_sbo_26_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_26\_sbc\_\_slash\_PP2B\_sbo\_26\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_26_sbc__slash_PP2B_sbo_26_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_26\_sbc\_\_slash\_PP2B\_sbo\_26\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_27_sbc__slash_PP2B_sbo_27_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_27\_sbc\_\_slash\_PP2B\_sbo\_27\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_27_sbc__slash_PP2B_sbo_27_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_27\_sbc\_\_slash\_PP2B\_sbo\_27\_sbc\_\_cplx}$ k1*AMPAR_slash_A835_star_845_star__B835_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_28_sbc__slash_PP2B_sbo_28_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_28\_sbc\_\_slash\_PP2B\_sbo\_28\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_28_sbc__slash_PP2B_sbo_28_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_28\_sbc\_\_slash\_PP2B\_sbo\_28\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_29_sbc__slash_PP2B_sbo_29_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_29\_sbc\_\_slash\_PP2B\_sbo\_29\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_29_sbc__slash_PP2B_sbo_29_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_29\_sbc\_\_slash\_PP2B\_sbo\_29\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_30_sbc__slash_PP2B_sbo_30_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_30\_sbc\_\_slash\_PP2B\_sbo\_30\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_30_sbc__slash_PP2B_sbo_30_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_30\_sbc\_\_slash\_PP2B\_sbo\_30\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_31_sbc__slash_PP2B_sbo_31_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_31\_sbc\_\_slash\_PP2B\_sbo\_31\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_31_sbc__slash_PP2B_sbo_31_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_31\_sbc\_\_slash\_PP2B\_sbo\_31\_sbc\_\_cplx}$ k1*AMPAR_slash_A835_star_845_star__B835_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_32_sbc__slash_PP2B_sbo_32_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_32\_sbc\_\_slash\_PP2B\_sbo\_32\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_32_sbc__slash_PP2B_sbo_32_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_32\_sbc\_\_slash\_PP2B\_sbo\_32\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_slash_PP2B_cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_slash\_PP2B\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_slash_PP2B_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_slash\_PP2B\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_1_sbc__slash_PP2B_sbo_1_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_1\_sbc\_\_slash\_PP2B\_sbo\_1\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_1_sbc__slash_PP2B_sbo_1_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_1\_sbc\_\_slash\_PP2B\_sbo\_1\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_2_sbc__slash_PP2B_sbo_2_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_2\_sbc\_\_slash\_PP2B\_sbo\_2\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_2_sbc__slash_PP2B_sbo_2_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_2\_sbc\_\_slash\_PP2B\_sbo\_2\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_3_sbc__slash_PP2B_sbo_3_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_3\_sbc\_\_slash\_PP2B\_sbo\_3\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_3_sbc__slash_PP2B_sbo_3_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_3\_sbc\_\_slash\_PP2B\_sbo\_3\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_4_sbc__slash_PP2B_sbo_4_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_4\_sbc\_\_slash\_PP2B\_sbo\_4\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_4_sbc__slash_PP2B_sbo_4_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_4\_sbc\_\_slash\_PP2B\_sbo\_4\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_5_sbc__slash_PP2B_sbo_5_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_5\_sbc\_\_slash\_PP2B\_sbo\_5\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_5_sbc__slash_PP2B_sbo_5_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_5\_sbc\_\_slash\_PP2B\_sbo\_5\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_6_sbc__slash_PP2B_sbo_6_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_6\_sbc\_\_slash\_PP2B\_sbo\_6\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_6_sbc__slash_PP2B_sbo_6_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_6\_sbc\_\_slash\_PP2B\_sbo\_6\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_7_sbc__slash_PP2B_sbo_7_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_7\_sbc\_\_slash\_PP2B\_sbo\_7\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_7_sbc__slash_PP2B_sbo_7_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_7\_sbc\_\_slash\_PP2B\_sbo\_7\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_8_sbc__slash_PP2B_sbo_8_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_8\_sbc\_\_slash\_PP2B\_sbo\_8\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_8_sbc__slash_PP2B_sbo_8_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_8\_sbc\_\_slash\_PP2B\_sbo\_8\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_9_sbc__slash_PP2B_sbo_9_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_9\_sbc\_\_slash\_PP2B\_sbo\_9\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_9_sbc__slash_PP2B_sbo_9_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_9\_sbc\_\_slash\_PP2B\_sbo\_9\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_10_sbc__slash_PP2B_sbo_10_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_10\_sbc\_\_slash\_PP2B\_sbo\_10\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_10_sbc__slash_PP2B_sbo_10_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_10\_sbc\_\_slash\_PP2B\_sbo\_10\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_11_sbc__slash_PP2B_sbo_11_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_11\_sbc\_\_slash\_PP2B\_sbo\_11\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_11_sbc__slash_PP2B_sbo_11_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_11\_sbc\_\_slash\_PP2B\_sbo\_11\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_12_sbc__slash_PP2B_sbo_12_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_12\_sbc\_\_slash\_PP2B\_sbo\_12\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_12_sbc__slash_PP2B_sbo_12_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_12\_sbc\_\_slash\_PP2B\_sbo\_12\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_13_sbc__slash_PP2B_sbo_13_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_13\_sbc\_\_slash\_PP2B\_sbo\_13\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_13_sbc__slash_PP2B_sbo_13_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_13\_sbc\_\_slash\_PP2B\_sbo\_13\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B831_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_14_sbc__slash_PP2B_sbo_14_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_14\_sbc\_\_slash\_PP2B\_sbo\_14\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_14_sbc__slash_PP2B_sbo_14_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_14\_sbc\_\_slash\_PP2B\_sbo\_14\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_845_star_*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_15_sbc__slash_PP2B_sbo_15_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_15\_sbc\_\_slash\_PP2B\_sbo\_15\_sbc\_\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_PP2B_sbo_15_sbc__slash_PP2B_sbo_15_sbc__cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_PP2B\_sbo\_15\_sbc\_\_slash\_PP2B\_sbo\_15\_sbc\_\_cplx}$ From Seki et al ABB 316(2):673-679 k1*CaM_slash_neurogranin_star__PSD*PP2B_slash_CaM_Ca_n_minus_CaNAB-k2*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_neurogranin_PSD_slash_dephosph_neurogranin_PSD_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin\_star\_\_PSD}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB}-\mathrm{k2}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_neurogranin\_PSD\_slash\_dephosph\_neurogranin\_PSD\_cplx}$ k3*PP2B_slash_CaM_Ca_n_minus_CaNAB_slash_dephosph_neurogranin_PSD_slash_dephosph_neurogranin_PSD_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaM\_Ca\_n\_minus\_CaNAB\_slash\_dephosph\_neurogranin\_PSD\_slash\_dephosph\_neurogranin\_PSD\_cplx}$ The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034 k1*PP1_slash_I1_star_*PP2B_slash_CaNAB_minus_Ca4-k2*PP2B_slash_CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_slash_dephosph_inhib1_noCaM_cplx $\mathrm{k1}\mathrm{PP1\_slash\_I1\_star\_}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4}-\mathrm{k2}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4\_slash\_dephosph\_inhib1\_noCaM\_slash\_dephosph\_inhib1\_noCaM\_cplx}$ k3*PP2B_slash_CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_slash_dephosph_inhib1_noCaM_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4\_slash\_dephosph\_inhib1\_noCaM\_slash\_dephosph\_inhib1\_noCaM\_cplx}$ The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034 k1*PP1_PSD_slash_I1_star_*PP2B_slash_CaNAB_minus_Ca4-k2*PP2B_slash_CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_PSD_slash_dephosph_inhib1_noCaM_PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_I1\_star\_}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4}-\mathrm{k2}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4\_slash\_dephosph\_inhib1\_noCaM\_PSD\_slash\_dephosph\_inhib1\_noCaM\_PSD\_cplx}$ k3*PP2B_slash_CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_PSD_slash_dephosph_inhib1_noCaM_PSD_cplx $\mathrm{k3}\mathrm{PP2B\_slash\_CaNAB\_minus\_Ca4\_slash\_dephosph\_inhib1\_noCaM\_PSD\_slash\_dephosph\_inhib1\_noCaM\_PSD\_cplx}$ Same rates as PKA-phosph-I1 k1*AC_slash_cAMP_minus_PDE*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_phosph_minus_PDE_slash_phosph_minus_PDE_cplx $\mathrm{k1}\mathrm{AC\_slash\_cAMP\_minus\_PDE}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_phosph\_minus\_PDE\_slash\_phosph\_minus\_PDE\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_phosph_minus_PDE_slash_phosph_minus_PDE_cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_phosph\_minus\_PDE\_slash\_phosph\_minus\_PDE\_cplx}$ #s from Bramson et al CRC crit rev Biochem 15:2 93-124. They have a huge list of peptide substrates and I have chosen high-ish rates. These consts give too much PKA activity, so lower Vmax 1/3. Now, k1 = 3e-5, k2 = 36, k3 = 9 (still pretty fast). Also lower Km 1/3 so k1 = 1e-5 Cohen et al FEBS Lett 76:182-86 1977 say rate =30% PKA act on phosphokinase beta. k1*PP1_slash_I1*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_minus_phosph_minus_I1_slash_PKA_minus_phosph_minus_I1_cplx $\mathrm{k1}\mathrm{PP1\_slash\_I1}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_minus\_phosph\_minus\_I1\_slash\_PKA\_minus\_phosph\_minus\_I1\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_minus_phosph_minus_I1_slash_PKA_minus_phosph_minus_I1_cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_minus\_phosph\_minus\_I1\_slash\_PKA\_minus\_phosph\_minus\_I1\_cplx}$ k1*PP1_PSD_slash_I1*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_minus_phosph_minus_I1_PSD_slash_PKA_minus_phosph_minus_I1_PSD_cplx $\mathrm{k1}\mathrm{PP1\_PSD\_slash\_I1}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_minus\_phosph\_minus\_I1\_PSD\_slash\_PKA\_minus\_phosph\_minus\_I1\_PSD\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_minus_phosph_minus_I1_PSD_slash_PKA_minus_phosph_minus_I1_PSD_cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_minus\_phosph\_minus\_I1\_PSD\_slash\_PKA\_minus\_phosph\_minus\_I1\_PSD\_cplx}$ k1*AMPAR_slash_A_B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_17_sbc__slash_PKA_sbo_17_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_17\_sbc\_\_slash\_PKA\_sbo\_17\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_17_sbc__slash_PKA_sbo_17_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_17\_sbc\_\_slash\_PKA\_sbo\_17\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_16_sbc__slash_PKA_sbo_16_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_16\_sbc\_\_slash\_PKA\_sbo\_16\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_16_sbc__slash_PKA_sbo_16_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_16\_sbc\_\_slash\_PKA\_sbo\_16\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_18_sbc__slash_PKA_sbo_18_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_18\_sbc\_\_slash\_PKA\_sbo\_18\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_18_sbc__slash_PKA_sbo_18_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_18\_sbc\_\_slash\_PKA\_sbo\_18\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_19_sbc__slash_PKA_sbo_19_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_19\_sbc\_\_slash\_PKA\_sbo\_19\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_19_sbc__slash_PKA_sbo_19_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_19\_sbc\_\_slash\_PKA\_sbo\_19\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_20_sbc__slash_PKA_sbo_20_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_20\_sbc\_\_slash\_PKA\_sbo\_20\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_20_sbc__slash_PKA_sbo_20_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_20\_sbc\_\_slash\_PKA\_sbo\_20\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_21_sbc__slash_PKA_sbo_21_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_21\_sbc\_\_slash\_PKA\_sbo\_21\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_21_sbc__slash_PKA_sbo_21_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_21\_sbc\_\_slash\_PKA\_sbo\_21\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_22_sbc__slash_PKA_sbo_22_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_22\_sbc\_\_slash\_PKA\_sbo\_22\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_22_sbc__slash_PKA_sbo_22_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_22\_sbc\_\_slash\_PKA\_sbo\_22\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_23_sbc__slash_PKA_sbo_23_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_23\_sbc\_\_slash\_PKA\_sbo\_23\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_23_sbc__slash_PKA_sbo_23_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_23\_sbc\_\_slash\_PKA\_sbo\_23\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_24_sbc__slash_PKA_sbo_24_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_24\_sbc\_\_slash\_PKA\_sbo\_24\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_24_sbc__slash_PKA_sbo_24_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_24\_sbc\_\_slash\_PKA\_sbo\_24\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_25_sbc__slash_PKA_sbo_25_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_25\_sbc\_\_slash\_PKA\_sbo\_25\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_25_sbc__slash_PKA_sbo_25_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_25\_sbc\_\_slash\_PKA\_sbo\_25\_sbc\_\_cplx}$ k1*AMPAR_slash_A_B831_star_845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_26_sbc__slash_PKA_sbo_26_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_26\_sbc\_\_slash\_PKA\_sbo\_26\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_26_sbc__slash_PKA_sbo_26_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_26\_sbc\_\_slash\_PKA\_sbo\_26\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star__B831_star_845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_27_sbc__slash_PKA_sbo_27_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_27\_sbc\_\_slash\_PKA\_sbo\_27\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_27_sbc__slash_PKA_sbo_27_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_27\_sbc\_\_slash\_PKA\_sbo\_27\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_28_sbc__slash_PKA_sbo_28_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_28\_sbc\_\_slash\_PKA\_sbo\_28\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_28_sbc__slash_PKA_sbo_28_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_28\_sbc\_\_slash\_PKA\_sbo\_28\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_29_sbc__slash_PKA_sbo_29_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_29\_sbc\_\_slash\_PKA\_sbo\_29\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_29_sbc__slash_PKA_sbo_29_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_29\_sbc\_\_slash\_PKA\_sbo\_29\_sbc\_\_cplx}$ k1*AMPAR_slash_A845_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_30_sbc__slash_PKA_sbo_30_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_30\_sbc\_\_slash\_PKA\_sbo\_30\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_30_sbc__slash_PKA_sbo_30_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_30\_sbc\_\_slash\_PKA\_sbo\_30\_sbc\_\_cplx}$ k1*AMPAR_slash_A831_star_845_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_31_sbc__slash_PKA_sbo_31_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_31\_sbc\_\_slash\_PKA\_sbo\_31\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_31_sbc__slash_PKA_sbo_31_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_31\_sbc\_\_slash\_PKA\_sbo\_31\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_slash_PKA_cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_slash\_PKA\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_slash_PKA_cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_slash\_PKA\_cplx}$ k1*AMPAR_memb_slash_A845_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_1_sbc__slash_PKA_sbo_1_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_1\_sbc\_\_slash\_PKA\_sbo\_1\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_1_sbc__slash_PKA_sbo_1_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_1\_sbc\_\_slash\_PKA\_sbo\_1\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_2_sbc__slash_PKA_sbo_2_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_2\_sbc\_\_slash\_PKA\_sbo\_2\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_2_sbc__slash_PKA_sbo_2_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_2\_sbc\_\_slash\_PKA\_sbo\_2\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_3_sbc__slash_PKA_sbo_3_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_3\_sbc\_\_slash\_PKA\_sbo\_3\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_3_sbc__slash_PKA_sbo_3_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_3\_sbc\_\_slash\_PKA\_sbo\_3\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_4_sbc__slash_PKA_sbo_4_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_4\_sbc\_\_slash\_PKA\_sbo\_4\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_4_sbc__slash_PKA_sbo_4_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_4\_sbc\_\_slash\_PKA\_sbo\_4\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_5_sbc__slash_PKA_sbo_5_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_5\_sbc\_\_slash\_PKA\_sbo\_5\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_5_sbc__slash_PKA_sbo_5_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_5\_sbc\_\_slash\_PKA\_sbo\_5\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_6_sbc__slash_PKA_sbo_6_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_6\_sbc\_\_slash\_PKA\_sbo\_6\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_6_sbc__slash_PKA_sbo_6_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_6\_sbc\_\_slash\_PKA\_sbo\_6\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_7_sbc__slash_PKA_sbo_7_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_7\_sbc\_\_slash\_PKA\_sbo\_7\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_7_sbc__slash_PKA_sbo_7_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_7\_sbc\_\_slash\_PKA\_sbo\_7\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_8_sbc__slash_PKA_sbo_8_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_8\_sbc\_\_slash\_PKA\_sbo\_8\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_8_sbc__slash_PKA_sbo_8_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_8\_sbc\_\_slash\_PKA\_sbo\_8\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_9_sbc__slash_PKA_sbo_9_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_9\_sbc\_\_slash\_PKA\_sbo\_9\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_9_sbc__slash_PKA_sbo_9_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_9\_sbc\_\_slash\_PKA\_sbo\_9\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_10_sbc__slash_PKA_sbo_10_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_10\_sbc\_\_slash\_PKA\_sbo\_10\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_10_sbc__slash_PKA_sbo_10_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_10\_sbc\_\_slash\_PKA\_sbo\_10\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_11_sbc__slash_PKA_sbo_11_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_11\_sbc\_\_slash\_PKA\_sbo\_11\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_11_sbc__slash_PKA_sbo_11_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_11\_sbc\_\_slash\_PKA\_sbo\_11\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_845_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_12_sbc__slash_PKA_sbo_12_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_12\_sbc\_\_slash\_PKA\_sbo\_12\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_12_sbc__slash_PKA_sbo_12_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_12\_sbc\_\_slash\_PKA\_sbo\_12\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_13_sbc__slash_PKA_sbo_13_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_13\_sbc\_\_slash\_PKA\_sbo\_13\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_13_sbc__slash_PKA_sbo_13_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_13\_sbc\_\_slash\_PKA\_sbo\_13\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_14_sbc__slash_PKA_sbo_14_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_14\_sbc\_\_slash\_PKA\_sbo\_14\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_14_sbc__slash_PKA_sbo_14_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_14\_sbc\_\_slash\_PKA\_sbo\_14\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_*PKA_slash_PKA_minus_active-k2*PKA_slash_PKA_minus_active_slash_PKA_sbo_15_sbc__slash_PKA_sbo_15_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PKA\_slash\_PKA\_minus\_active}-\mathrm{k2}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_15\_sbc\_\_slash\_PKA\_sbo\_15\_sbc\_\_cplx}$ k3*PKA_slash_PKA_minus_active_slash_PKA_sbo_15_sbc__slash_PKA_sbo_15_sbc__cplx $\mathrm{k3}\mathrm{PKA\_slash\_PKA\_minus\_active\_slash\_PKA\_sbo\_15\_sbc\_\_slash\_PKA\_sbo\_15\_sbc\_\_cplx}$ 17 Feb 2005 Halved Vmax as the amount of enzyme has been doubled to get an integer value in the spine. 18 Feb 2005. Updated Km from BRENDA: EC No 4.6.1.1 Rat Km: 0.95 mM Turnover 12/sec. Human Km: 0.3 mM 12 umol/min/mg for mammalia, turnover is 12/sec See PMID 8663304 by Dessauer and Gilman JBC 271 1996 Unfortunately turnover range is from 34 down to 0.1 in different studies, according to BRENDA. kcat*AC_slash_AC1_minus_CaM*AC_slash_ATP/(Km+AC_slash_ATP) $\frac{\mathrm{kcat}\mathrm{AC\_slash\_AC1\_minus\_CaM}\mathrm{AC\_slash\_ATP}}{\mathrm{Km}+\mathrm{AC\_slash\_ATP}}$ Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18 18 Feb: Raised Km to 300 based on BRENDA data. Unlikely to make much difference, given the vast amount of ATP. kcat*AC_slash_AC2_star_*AC_slash_ATP/(Km+AC_slash_ATP) $\frac{\mathrm{kcat}\mathrm{AC\_slash\_AC2\_star\_}\mathrm{AC\_slash\_ATP}}{\mathrm{Km}+\mathrm{AC\_slash\_ATP}}$ Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6 k1*cAMP*AC_slash_cAMP_minus_PDE-k2*AC_slash_cAMP_minus_PDE_slash_PDE_slash_PDE_cplx $\mathrm{k1}\mathrm{cAMP}\mathrm{AC\_slash\_cAMP\_minus\_PDE}-\mathrm{k2}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_slash\_PDE\_slash\_PDE\_cplx}$ k3*AC_slash_cAMP_minus_PDE_slash_PDE_slash_PDE_cplx $\mathrm{k3}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_slash\_PDE\_slash\_PDE\_cplx}$ This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We\'ll ignore cGMP effects for now. k1*cAMP*AC_slash_cAMP_minus_PDE_star_-k2*AC_slash_cAMP_minus_PDE_star__slash_PDE_star__slash_PDE_star__cplx $\mathrm{k1}\mathrm{cAMP}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_star\_}-\mathrm{k2}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_star\_\_slash\_PDE\_star\_\_slash\_PDE\_star\_\_cplx}$ k3*AC_slash_cAMP_minus_PDE_star__slash_PDE_star__slash_PDE_star__cplx $\mathrm{k3}\mathrm{AC\_slash\_cAMP\_minus\_PDE\_star\_\_slash\_PDE\_star\_\_slash\_PDE\_star\_\_cplx}$ Rate is 1/6 of the CaM stim form. We\'ll just reduce all lf k1, k2, k3 so that the Vmax goes down 1/6. k1*cAMP*AC_slash_PDE1-k2*AC_slash_PDE1_slash_PDE1_slash_PDE1_cplx $\mathrm{k1}\mathrm{cAMP}\mathrm{AC\_slash\_PDE1}-\mathrm{k2}\mathrm{AC\_slash\_PDE1\_slash\_PDE1\_slash\_PDE1\_cplx}$ k3*AC_slash_PDE1_slash_PDE1_slash_PDE1_cplx $\mathrm{k3}\mathrm{AC\_slash\_PDE1\_slash\_PDE1\_slash\_PDE1\_cplx}$ Max activity ~10umol/min/mg in presence of lots of CaM. Affinity is low, 40 uM. k3 = 10, k2 = 40, k1 = (50/40) / 6e5. k1*cAMP*AC_slash_CaM_dot_PDE1-k2*AC_slash_CaM_dot_PDE1_slash_CaM_dot_PDE1_slash_CaM_dot_PDE1_cplx $\mathrm{k1}\mathrm{cAMP}\mathrm{AC\_slash\_CaM\_dot\_PDE1}-\mathrm{k2}\mathrm{AC\_slash\_CaM\_dot\_PDE1\_slash\_CaM\_dot\_PDE1\_slash\_CaM\_dot\_PDE1\_cplx}$ k3*AC_slash_CaM_dot_PDE1_slash_CaM_dot_PDE1_slash_CaM_dot_PDE1_cplx $\mathrm{k3}\mathrm{AC\_slash\_CaM\_dot\_PDE1\_slash\_CaM\_dot\_PDE1\_slash\_CaM\_dot\_PDE1\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_slash_PP1_cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_slash\_PP1\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_slash_PP1_cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_slash\_PP1\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_1_sbc__slash_PP1_sbo_1_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_1\_sbc\_\_slash\_PP1\_sbo\_1\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_1_sbc__slash_PP1_sbo_1_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_1\_sbc\_\_slash\_PP1\_sbo\_1\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_2_sbc__slash_PP1_sbo_2_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_2\_sbc\_\_slash\_PP1\_sbo\_2\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_2_sbc__slash_PP1_sbo_2_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_2\_sbc\_\_slash\_PP1\_sbo\_2\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_3_sbc__slash_PP1_sbo_3_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_3\_sbc\_\_slash\_PP1\_sbo\_3\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_3_sbc__slash_PP1_sbo_3_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_3\_sbc\_\_slash\_PP1\_sbo\_3\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_4_sbc__slash_PP1_sbo_4_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_4\_sbc\_\_slash\_PP1\_sbo\_4\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_4_sbc__slash_PP1_sbo_4_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_4\_sbc\_\_slash\_PP1\_sbo\_4\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_5_sbc__slash_PP1_sbo_5_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_5\_sbc\_\_slash\_PP1\_sbo\_5\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_5_sbc__slash_PP1_sbo_5_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_5\_sbc\_\_slash\_PP1\_sbo\_5\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_6_sbc__slash_PP1_sbo_6_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_6\_sbc\_\_slash\_PP1\_sbo\_6\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_6_sbc__slash_PP1_sbo_6_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_6\_sbc\_\_slash\_PP1\_sbo\_6\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_7_sbc__slash_PP1_sbo_7_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_7\_sbc\_\_slash\_PP1\_sbo\_7\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_7_sbc__slash_PP1_sbo_7_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_7\_sbc\_\_slash\_PP1\_sbo\_7\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_8_sbc__slash_PP1_sbo_8_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_8\_sbc\_\_slash\_PP1\_sbo\_8\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_8_sbc__slash_PP1_sbo_8_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_8\_sbc\_\_slash\_PP1\_sbo\_8\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_9_sbc__slash_PP1_sbo_9_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_9\_sbc\_\_slash\_PP1\_sbo\_9\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_9_sbc__slash_PP1_sbo_9_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_9\_sbc\_\_slash\_PP1\_sbo\_9\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_10_sbc__slash_PP1_sbo_10_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_10\_sbc\_\_slash\_PP1\_sbo\_10\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_10_sbc__slash_PP1_sbo_10_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_10\_sbc\_\_slash\_PP1\_sbo\_10\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_11_sbc__slash_PP1_sbo_11_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_11\_sbc\_\_slash\_PP1\_sbo\_11\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_11_sbc__slash_PP1_sbo_11_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_11\_sbc\_\_slash\_PP1\_sbo\_11\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_12_sbc__slash_PP1_sbo_12_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_12\_sbc\_\_slash\_PP1\_sbo\_12\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_12_sbc__slash_PP1_sbo_12_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_12\_sbc\_\_slash\_PP1\_sbo\_12\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_13_sbc__slash_PP1_sbo_13_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_13\_sbc\_\_slash\_PP1\_sbo\_13\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_13_sbc__slash_PP1_sbo_13_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_13\_sbc\_\_slash\_PP1\_sbo\_13\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_14_sbc__slash_PP1_sbo_14_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_14\_sbc\_\_slash\_PP1\_sbo\_14\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_14_sbc__slash_PP1_sbo_14_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_14\_sbc\_\_slash\_PP1\_sbo\_14\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_15_sbc__slash_PP1_sbo_15_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_15\_sbc\_\_slash\_PP1\_sbo\_15\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_15_sbc__slash_PP1_sbo_15_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_15\_sbc\_\_slash\_PP1\_sbo\_15\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_16_sbc__slash_PP1_sbo_16_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_16\_sbc\_\_slash\_PP1\_sbo\_16\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_16_sbc__slash_PP1_sbo_16_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_16\_sbc\_\_slash\_PP1\_sbo\_16\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_17_sbc__slash_PP1_sbo_17_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_17\_sbc\_\_slash\_PP1\_sbo\_17\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_17_sbc__slash_PP1_sbo_17_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_17\_sbc\_\_slash\_PP1\_sbo\_17\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_18_sbc__slash_PP1_sbo_18_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_18\_sbc\_\_slash\_PP1\_sbo\_18\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_18_sbc__slash_PP1_sbo_18_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_18\_sbc\_\_slash\_PP1\_sbo\_18\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_19_sbc__slash_PP1_sbo_19_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_19\_sbc\_\_slash\_PP1\_sbo\_19\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_19_sbc__slash_PP1_sbo_19_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_19\_sbc\_\_slash\_PP1\_sbo\_19\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_20_sbc__slash_PP1_sbo_20_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_20\_sbc\_\_slash\_PP1\_sbo\_20\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_20_sbc__slash_PP1_sbo_20_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_20\_sbc\_\_slash\_PP1\_sbo\_20\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_21_sbc__slash_PP1_sbo_21_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_21\_sbc\_\_slash\_PP1\_sbo\_21\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_21_sbc__slash_PP1_sbo_21_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_21\_sbc\_\_slash\_PP1\_sbo\_21\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_22_sbc__slash_PP1_sbo_22_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_22\_sbc\_\_slash\_PP1\_sbo\_22\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_22_sbc__slash_PP1_sbo_22_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_22\_sbc\_\_slash\_PP1\_sbo\_22\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_23_sbc__slash_PP1_sbo_23_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_23\_sbc\_\_slash\_PP1\_sbo\_23\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_23_sbc__slash_PP1_sbo_23_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_23\_sbc\_\_slash\_PP1\_sbo\_23\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_24_sbc__slash_PP1_sbo_24_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_24\_sbc\_\_slash\_PP1\_sbo\_24\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_24_sbc__slash_PP1_sbo_24_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_24\_sbc\_\_slash\_PP1\_sbo\_24\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_25_sbc__slash_PP1_sbo_25_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_25\_sbc\_\_slash\_PP1\_sbo\_25\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_25_sbc__slash_PP1_sbo_25_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_25\_sbc\_\_slash\_PP1\_sbo\_25\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_26_sbc__slash_PP1_sbo_26_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_26\_sbc\_\_slash\_PP1\_sbo\_26\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_26_sbc__slash_PP1_sbo_26_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_26\_sbc\_\_slash\_PP1\_sbo\_26\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_27_sbc__slash_PP1_sbo_27_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_27\_sbc\_\_slash\_PP1\_sbo\_27\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_27_sbc__slash_PP1_sbo_27_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_27\_sbc\_\_slash\_PP1\_sbo\_27\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A835_star_845_star__B835_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_28_sbc__slash_PP1_sbo_28_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A835\_star\_845\_star\_\_B835\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_28\_sbc\_\_slash\_PP1\_sbo\_28\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_28_sbc__slash_PP1_sbo_28_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_28\_sbc\_\_slash\_PP1\_sbo\_28\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_845_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_29_sbc__slash_PP1_sbo_29_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_845\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_29\_sbc\_\_slash\_PP1\_sbo\_29\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_29_sbc__slash_PP1_sbo_29_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_29\_sbc\_\_slash\_PP1\_sbo\_29\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_30_sbc__slash_PP1_sbo_30_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_30\_sbc\_\_slash\_PP1\_sbo\_30\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_30_sbc__slash_PP1_sbo_30_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_30\_sbc\_\_slash\_PP1\_sbo\_30\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B831_star_*PP1_PSD_slash_PP1_minus_active_PSD-k2*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_31_sbc__slash_PP1_sbo_31_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B831\_star\_}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD}-\mathrm{k2}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_31\_sbc\_\_slash\_PP1\_sbo\_31\_sbc\_\_cplx}$ k3*PP1_PSD_slash_PP1_minus_active_PSD_slash_PP1_sbo_31_sbc__slash_PP1_sbo_31_sbc__cplx $\mathrm{k3}\mathrm{PP1\_PSD\_slash\_PP1\_minus\_active\_PSD\_slash\_PP1\_sbo\_31\_sbc\_\_slash\_PP1\_sbo\_31\_sbc\_\_cplx}$ Rates from Huang et al ABB 305:2 570-580 1993 k1*CaM_slash_neurogranin*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_slash_PKC_minus_phosph_minus_neurogranin_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin}\mathrm{PKC\_minus\_active}-\mathrm{k2}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_cplx}$ k3*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_slash_PKC_minus_phosph_minus_neurogranin_cplx $\mathrm{k3}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_cplx}$ Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 k1*CaM_slash_neurogranin_minus_CaM*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_slash_PKC_minus_phosph_minus_ng_minus_CaM_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin\_minus\_CaM}\mathrm{PKC\_minus\_active}-\mathrm{k2}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_cplx}$ k3*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_slash_PKC_minus_phosph_minus_ng_minus_CaM_cplx $\mathrm{k3}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_cplx}$ Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4 k1*AC_slash_AC2*PKC_minus_active-k2*PKC_minus_active_slash_phosph_minus_AC2_slash_phosph_minus_AC2_cplx $\mathrm{k1}\mathrm{AC\_slash\_AC2}\mathrm{PKC\_minus\_active}-\mathrm{k2}\mathrm{PKC\_minus\_active\_slash\_phosph\_minus\_AC2\_slash\_phosph\_minus\_AC2\_cplx}$ k3*PKC_minus_active_slash_phosph_minus_AC2_slash_phosph_minus_AC2_cplx $\mathrm{k3}\mathrm{PKC\_minus\_active\_slash\_phosph\_minus\_AC2\_slash\_phosph\_minus\_AC2\_cplx}$ Rates from Huang et al ABB 305:2 570-580 1993 k1*CaM_slash_neurogranin_PSD*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_PSD_slash_PKC_minus_phosph_minus_neurogranin_PSD_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin\_PSD}\mathrm{PKC\_minus\_active}-\mathrm{k2}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_PSD\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_PSD\_cplx}$ k3*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_PSD_slash_PKC_minus_phosph_minus_neurogranin_PSD_cplx $\mathrm{k3}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_PSD\_slash\_PKC\_minus\_phosph\_minus\_neurogranin\_PSD\_cplx}$ Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 k1*CaM_slash_neurogranin_minus_CaM_PSD*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_PSD_slash_PKC_minus_phosph_minus_ng_minus_CaM_PSD_cplx $\mathrm{k1}\mathrm{CaM\_slash\_neurogranin\_minus\_CaM\_PSD}\mathrm{PKC\_minus\_active}-\mathrm{k2}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_PSD\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_PSD\_cplx}$ k3*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_PSD_slash_PKC_minus_phosph_minus_ng_minus_CaM_PSD_cplx $\mathrm{k3}\mathrm{PKC\_minus\_active\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_PSD\_slash\_PKC\_minus\_phosph\_minus\_ng\_minus\_CaM\_PSD\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_CaM_minus_PSD*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD-k2*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_CaM\_minus\_PSD}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD}-\mathrm{k2}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286\_slash\_Deph\_minus\_thr286\_cplx}$ k3*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx $\mathrm{k3}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286\_slash\_Deph\_minus\_thr286\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_PSD*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD-k2*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286b_slash_Deph_minus_thr286b_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD}-\mathrm{k2}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286b\_slash\_Deph\_minus\_thr286b\_cplx}$ k3*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286b_slash_Deph_minus_thr286b_cplx $\mathrm{k3}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286b\_slash\_Deph\_minus\_thr286b\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_star__star__star__minus_PSD*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD-k2*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_star\_\_star\_\_star\_\_minus\_PSD}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD}-\mathrm{k2}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr305\_slash\_Deph\_minus\_thr305\_cplx}$ k3*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx $\mathrm{k3}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr305\_slash\_Deph\_minus\_thr305\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_star__star__star__minus_PSD*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD-k2*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_star\_\_star\_\_star\_\_minus\_PSD}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD}-\mathrm{k2}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286c\_slash\_Deph\_minus\_thr286c\_cplx}$ k3*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx $\mathrm{k3}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr286c\_slash\_Deph\_minus\_thr286c\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_thr305_minus_PSD*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD-k2*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr305a_slash_Deph_minus_thr305a_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr305\_minus\_PSD}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD}-\mathrm{k2}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr305a\_slash\_Deph\_minus\_thr305a\_cplx}$ k3*PP1_CaMKII_PSD_slash_PP1_minus_active_CaMKII_PSD_slash_Deph_minus_thr305a_slash_Deph_minus_thr305a_cplx $\mathrm{k3}\mathrm{PP1\_CaMKII\_PSD\_slash\_PP1\_minus\_active\_CaMKII\_PSD\_slash\_Deph\_minus\_thr305a\_slash\_Deph\_minus\_thr305a\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_PSD*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_minus_act_minus_305_minus_PSD_slash_CaM_minus_act_minus_305_minus_PSD_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_305\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_305\_minus\_PSD\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_minus_act_minus_305_minus_PSD_slash_CaM_minus_act_minus_305_minus_PSD_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_305\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_305\_minus\_PSD\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_CaM_minus_PSD*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_minus_act_minus_286_minus_PSD_slash_CaM_minus_act_minus_286_minus_PSD_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_286\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_286\_minus\_PSD\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_minus_act_minus_286_minus_PSD_slash_CaM_minus_act_minus_286_minus_PSD_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_286\_minus\_PSD\_slash\_CaM\_minus\_act\_minus\_286\_minus\_PSD\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_PSD*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_act_autoph_slash_CaM_act_autoph_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_act\_autoph\_slash\_CaM\_act\_autoph\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_CaM_minus_CaMKII_minus_PSD_slash_CaM_act_autoph_slash_CaM_act_autoph_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_CaM\_minus\_CaMKII\_minus\_PSD\_slash\_CaM\_act\_autoph\_slash\_CaM\_act\_autoph\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_thr286_minus_PSD*CaMKII_PSD_slash_tot_minus_auto_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_305_minus_PSD_slash_auton_minus_305_minus_PSD_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_thr286\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_305\_minus\_PSD\_slash\_auton\_minus\_305\_minus\_PSD\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_305_minus_PSD_slash_auton_minus_305_minus_PSD_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_305\_minus\_PSD\_slash\_auton\_minus\_305\_minus\_PSD\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_CaM_minus_PSD*CaMKII_PSD_slash_tot_minus_auto_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_286_minus_PSD_slash_auton_minus_286_minus_PSD_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_CaM\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_286\_minus\_PSD\_slash\_auton\_minus\_286\_minus\_PSD\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_286_minus_PSD_slash_auton_minus_286_minus_PSD_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_286\_minus\_PSD\_slash\_auton\_minus\_286\_minus\_PSD\_cplx}$ k1*CaMKII_PSD_slash_CaMKII_minus_PSD*CaMKII_PSD_slash_tot_minus_auto_minus_PSD-k2*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_autoph_slash_auton_minus_autoph_cplx $\mathrm{k1}\mathrm{CaMKII\_PSD\_slash\_CaMKII\_minus\_PSD}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_autoph\_slash\_auton\_minus\_autoph\_cplx}$ k3*CaMKII_PSD_slash_tot_minus_auto_minus_PSD_slash_auton_minus_autoph_slash_auton_minus_autoph_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_tot\_minus\_auto\_minus\_PSD\_slash\_auton\_minus\_autoph\_slash\_auton\_minus\_autoph\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_slash_CaMKII_cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_slash\_CaMKII\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_slash_CaMKII_cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_slash\_CaMKII\_cplx}$ k1*AMPAR_memb_slash_A_B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_1_sbc__slash_CaMKII_sbo_1_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_1\_sbc\_\_slash\_CaMKII\_sbo\_1\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_1_sbc__slash_CaMKII_sbo_1_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_1\_sbc\_\_slash\_CaMKII\_sbo\_1\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_2_sbc__slash_CaMKII_sbo_2_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_2\_sbc\_\_slash\_CaMKII\_sbo\_2\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_2_sbc__slash_CaMKII_sbo_2_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_2\_sbc\_\_slash\_CaMKII\_sbo\_2\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_3_sbc__slash_CaMKII_sbo_3_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_3\_sbc\_\_slash\_CaMKII\_sbo\_3\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_3_sbc__slash_CaMKII_sbo_3_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_3\_sbc\_\_slash\_CaMKII\_sbo\_3\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_4_sbc__slash_CaMKII_sbo_4_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_4\_sbc\_\_slash\_CaMKII\_sbo\_4\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_4_sbc__slash_CaMKII_sbo_4_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_4\_sbc\_\_slash\_CaMKII\_sbo\_4\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_5_sbc__slash_CaMKII_sbo_5_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_5\_sbc\_\_slash\_CaMKII\_sbo\_5\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_5_sbc__slash_CaMKII_sbo_5_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_5\_sbc\_\_slash\_CaMKII\_sbo\_5\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_6_sbc__slash_CaMKII_sbo_6_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_6\_sbc\_\_slash\_CaMKII\_sbo\_6\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_6_sbc__slash_CaMKII_sbo_6_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_6\_sbc\_\_slash\_CaMKII\_sbo\_6\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_7_sbc__slash_CaMKII_sbo_7_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_7\_sbc\_\_slash\_CaMKII\_sbo\_7\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_7_sbc__slash_CaMKII_sbo_7_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_7\_sbc\_\_slash\_CaMKII\_sbo\_7\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_8_sbc__slash_CaMKII_sbo_8_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_8\_sbc\_\_slash\_CaMKII\_sbo\_8\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_8_sbc__slash_CaMKII_sbo_8_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_8\_sbc\_\_slash\_CaMKII\_sbo\_8\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_9_sbc__slash_CaMKII_sbo_9_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_9\_sbc\_\_slash\_CaMKII\_sbo\_9\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_9_sbc__slash_CaMKII_sbo_9_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_9\_sbc\_\_slash\_CaMKII\_sbo\_9\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A845_star__B831_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_10_sbc__slash_CaMKII_sbo_10_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A845\_star\_\_B831\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_10\_sbc\_\_slash\_CaMKII\_sbo\_10\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_10_sbc__slash_CaMKII_sbo_10_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_10\_sbc\_\_slash\_CaMKII\_sbo\_10\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A_B831_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_11_sbc__slash_CaMKII_sbo_11_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A\_B831\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_11\_sbc\_\_slash\_CaMKII\_sbo\_11\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_11_sbc__slash_CaMKII_sbo_11_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_11\_sbc\_\_slash\_CaMKII\_sbo\_11\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_12_sbc__slash_CaMKII_sbo_12_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_12\_sbc\_\_slash\_CaMKII\_sbo\_12\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_12_sbc__slash_CaMKII_sbo_12_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_12\_sbc\_\_slash\_CaMKII\_sbo\_12\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B845_star_*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_13_sbc__slash_CaMKII_sbo_13_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B845\_star\_}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_13\_sbc\_\_slash\_CaMKII\_sbo\_13\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_13_sbc__slash_CaMKII_sbo_13_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_13\_sbc\_\_slash\_CaMKII\_sbo\_13\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star_845_star__B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_14_sbc__slash_CaMKII_sbo_14_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_845\_star\_\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_14\_sbc\_\_slash\_CaMKII\_sbo\_14\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_14_sbc__slash_CaMKII_sbo_14_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_14\_sbc\_\_slash\_CaMKII\_sbo\_14\_sbc\_\_cplx}$ k1*AMPAR_memb_slash_A831_star__B*CaMKII_PSD_slash_actCaMKII_minus_PSD-k2*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_15_sbc__slash_CaMKII_sbo_15_sbc__cplx $\mathrm{k1}\mathrm{AMPAR\_memb\_slash\_A831\_star\_\_B}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD}-\mathrm{k2}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_15\_sbc\_\_slash\_CaMKII\_sbo\_15\_sbc\_\_cplx}$ k3*CaMKII_PSD_slash_actCaMKII_minus_PSD_slash_CaMKII_sbo_15_sbc__slash_CaMKII_sbo_15_sbc__cplx $\mathrm{k3}\mathrm{CaMKII\_PSD\_slash\_actCaMKII\_minus\_PSD\_slash\_CaMKII\_sbo\_15\_sbc\_\_slash\_CaMKII\_sbo\_15\_sbc\_\_cplx}$