This model is taken from the <a href = "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=10493814&query_hl=10&itool=pubmed_docsum">Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51</a>. This model shows kinetic binding of NOHArginine to neuronal nitric oxide synthase. Model shows the substrate (NOH-Arginine) binding to nNOS in a two-step reversible fashion. First there is rapid binding equilibrium between Im-nNOS and NOH-Arginine to form an intermediate that contains bound imidazole and NOH-arginine. This is followed by a slower conformational change in the Im-enzyme-substrate complex that is associated with release of bound imidazole and generation of a modified enzyme-substrate complex which is detected due to spectral change. Rates approximated based on data in Table 2. The rates for first reaction are not exact since only Kd known and appropriate graphs do not exist for matching the profile. Wed Oct 18 16:58:47 2006 pathway Sharat J. Vayttaden and Prasoon Agarwal, NCBS Abu-Soud HM, Wang J, Rousseau DL, and Stuehr DJ Human Kidney cell expressing the rat brain nNOS Cytosol Quantitative match to experiments Exact GENESIS implementation Approximates original data Release of Imidazole from complex of NOH-Arginine and Imidazole bound rat brain neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 21;38(38):12446-51. kf*Im_minus_nNOS_minus_NOH_minus_Arginine-kb*nNOS_minus_NOH_minus_Arginine*Imidazole $\mathrm{kf}\mathrm{Im\_minus\_nNOS\_minus\_NOH\_minus\_Arginine}-\mathrm{kb}\mathrm{nNOS\_minus\_NOH\_minus\_Arginine}\mathrm{Imidazole}$ Binding of NOH-Arginine to Imidazole bound rat brain neuronal Nitric Oxide Synthase. Only Kd is known, Kf and Kb need to be approximated accordingly to match kinetic profiles. Due to lack of appropriate graphs the choice of Kf and Kb here is arbitrary and matches only the Kd. Kd = 2.7mM = 2700uM Abu-Soud HM et al. Biochemistry. 1999 21;38(38):12446-51. kf*NOH_minus_Arginine*Im_minus_nNOS-kb*Im_minus_nNOS_minus_NOH_minus_Arginine $\mathrm{kf}\mathrm{NOH\_minus\_Arginine}\mathrm{Im\_minus\_nNOS}-\mathrm{kb}\mathrm{Im\_minus\_nNOS\_minus\_NOH\_minus\_Arginine}$