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Molecule Parameter List for PKC-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000040

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • NonOsc_Ca_
    IP3metabolism
  • 31Network
    MIPP CaMKII CaM 
    PKC IP3-3K CaRegulation 
    Gq PLCbeta 134_dephos 
    145_dephos IP4-system IHP-system 
    1345_dephos 
    This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    PKC-cytosolic acting as a Molecule in  
    NonOsc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PKC-cytosolic
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PKC
    Pathway No. : 147
    11000No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells Kikkawa et al 1982 JBC 257(22):13341 have PKC levels in brain at about 1 uM. The cytosolic form is the inactive PKC. This is really a composite of three isoforms: alpha, beta and gamma which have slightly different properties and respond to different combinations of Ca, AA and DAG.

    PKC-cytosolic acting as a Substrate in a reaction in  
    NonOsc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-Ca
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PKC
    Pathway No. : 147
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      This Kd is a straightforward result from the Schaechter and Benowitz 1993 J Neurosci 13(10):4361 curves. The time-course is based on the known rapid activation of PKC and also the fact that Ca association with proteins is typically quite fast. My guess is that this tau of 2 sec is quite conservative and the actualy rate may be much faster. The parameter is quite insensitive for most stimuli.
    2PKC-basal-act
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PKC
    Pathway No. : 147
    1
    (s^-1)
    50
    (s^-1)
    Keq = 50(uM)0.02secSubstrate
    PKC-cytosolic

    Product
    PKC-basal*
      Basal activity of PKC is quite high, about 10% of max. See Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and Shinomura et al 1991 PNAS 88:5149-5153. This is partly due to basal levels of DAG, AA and Ca, but even when these are taken into account (see the derivations as per the PKC general notes) there is a small basal activity still to be accounted for. This reaction handles it by giving a 2% activity at baseline.
    3PKC-act-by-AA
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PKC
    Pathway No. : 147
    0.0001
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 833.3333(uM)-Substrate
    AA
    PKC-cytosolic

    Product
    PKC-AA*
      AA stimulates PKC activity even at rather low Ca. Schaechter and Benowitz 1993 J Neurosci 13(10):4361 Note that this one reaction combines the initial interaction and also membrane translocation.
    4PKC-n-DAG
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PKC
    Pathway No. : 147
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6667(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      Binding of PKC to DAG, non-Ca dependent. Kf based on Shinomura et al PNAS 88 5149-5153 1991 Tau estimated as fast and here it is about the same time-course as the formation of DAG so it will not be rate-limiting.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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