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Molecule Parameter List for IP3_3K_CaM* | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 32 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
IP3_3K_CaM* acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | IP3_3K_CaM* | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP3-3K
Pathway No. : 162 | 0 | 1000 | No | | 8-10 fold activation with both CaM bound and CaMKII phosphorylation (phos at thr311) Communi et al; EMBO J 16 (8), pp 1943-1952, 1997 |
IP3_3K_CaM* acting as a Product of an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | tot_CaM_CaMKII /
CaM-CaMK-phos1 | Osc_Ca_
IP3metabolism
Accession No. : 32 | CaMKII
Pathway No. : 159 | 1.59998 | 0.5 | 4 | explicit E-S complex | Substrate
IP3_3K_CaM
Product
IP3_3K_CaM*
| | | rates referred from standard CaM-CaMKII phosphorylation rates | | 2 | tot_autonomous_
CaMKII /
CaMK-phos1 | Osc_Ca_
IP3metabolism
Accession No. : 32 | CaMKII
Pathway No. : 159 | 2.49995 | 0.5 | 4 | explicit E-S complex | Substrate
IP3_3K_CaM
Product
IP3_3K_CaM*
| | | rates referred from standard CaMKII phosphorylation rates |
IP3_3K_CaM* acting as a Substrate in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 3k-CaM*-on | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP3-3K
Pathway No. : 162 | 80.358
(uM^-1 s^-1) | 180
(s^-1) | Kd(bf) = 2.24(uM) | - | Substrate
IP3(145)
IP3_3K_CaM*
Product
3kCaM*_ip3_
cmplx
| | Rates from Km of enzyme Communi et al, EMBO J 16(8) |
IP3_3K_CaM* acting as a Product in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | 3K*-bind-CaM | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP3-3K
Pathway No. : 162 | 49.9998
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.002(uM) | - | Substrate
CaM-Ca4
IP3_3K*
Product
IP3_3K_CaM*
| | | Communi et al, EMBO J 16; 1997 phosphorylated 3kinase has 25 fold greater sensitivity to CaM binding than the non-phosphorylated enzyme (Kd of 2nM) | | 2 | 3k-CaM*-off | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP3-3K
Pathway No. : 162 | 45
(s^-1) | 0.4148
(uM^-1 s^-1) | Kd(cb) = 0.0092(uM) | - | Substrate
3kCaM*_ip3_
cmplx
Product
IP3_3K_CaM*
IP4(1345)
| | | Kf = Vmax for enzyme (Communi et al, EMBO J 16(8)) Vmax is such that enzyme activity is 9 fold above basal. Kb derived from Keq value when reaction free energy = -10 kJ/mol |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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