DOQCS database

NCBS Home page

Accession List

Pathway List

Authorized Users

Help

News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.

Molecule Parameter List for R2C2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*R2C2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
fig3_CaMKII	2	Network	Shared_Object_fig3_CaMKII, CaMKII, CaM, PP1, PP2B, PKA, AC
This is the model file for figure 3 from Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It is a model of the Ca activation of CaMKII and other CaM-activated enzymes. It includes the regulatory phosphatases PP1 and PP2B (Calcineurin) acting on CaMKII and also includes CaM-activated adenylyl cyclase and PKA in the synapse. Demonstration script files for generating the figures in the paper, including figure 3, are available here.

R2C2 acting as a Molecule in fig3_CaMKII Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
R2C2	fig3_CaMKII Accession No. : 2	PKA Pathway No. : 17	0.5	1000	No
PKA: Protein kinase activated by cAMP. R2C2 complex consists of 2 catalytic (C) subunits, and the R-dimer. There are two types of cAMP binding sites termed A and B according to the rate with which they exchange bound labelled nucleotide. Dagfinn Ogreid and Stein Ove Doskeland (1981) Int.J.Biochem. 13(1):1-19 Physiological concentration of PKA = 0.59 uM (cardiac myocyte) Jeffrey J. Saucerman et al (2003) J.Biol.Chem. 278(48):47997-48003

R2C2 acting as a Substrate in a reaction in fig3_CaMKII Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
cAMP-bind-site-B 1	fig3_CaMKII Accession No. : 2	PKA Pathway No. : 17	54 (uM^-1 s^-1)	33 (s^-1)	Kd(bf) = 0.6111(uM)	-	Substrate R2C2 cAMP Product R2C2-cAMP
Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741 Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595 Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.