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Molecule Parameter List for PLC-Ca-Gq

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLC-Ca-Gq participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010012

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Osc_Ca_
    IP3metabolism
  • 32Network
    MIPP CaMKII CaM 
    PKC IP3-3K Gq 
    PLCbeta 134_dephos 145_dephos 
    IP4-system IHP-system 1345_dephos 
    CaRegulation Othmer-Tang-model 
    This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    PLC-Ca-Gq acting as a Molecule in  
    Osc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLC-Ca-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    01000No
    This should really be labelled PLC-Ca-GTP.Gq_alpha This is the most active form of the enzyme.

    PLC-Ca-Gq acting as an Enzyme in  
    Osc_Ca_IP3metabolism Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PLC-Ca-Gq /
    PLCb-Ca-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    4.999941604explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
    Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991

    PLC-Ca-Gq acting as a Substrate in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    Inact-PLC-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    1.6667
    (s^-1)
    0
    (uM^-1 s^-1)
    --Substrate
    PLC-Ca-Gq

    Product
    G*GDP
    PLC-Ca
    Rate of 100/min to account for GAP activity of PLC: Biddlecome et al, JBC, 271, 14, 7999-8007, 1996

    PLC-Ca-Gq acting as a Product in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PLC-Gq-bind-Ca
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    30
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0333(uM)-Substrate
    Ca
    PLC-Gq

    Product
    PLC-Ca-Gq
      this step has a high affinity for Ca, from Smrcka et al. 0.1uM so kf /kb = 1/6e4 = 1.666e-5:1. See the Act-PLC-by-Gq reaction. Raised kf to 5e-5 based on match to conc-eff curves from Smrcka et al.
    2Act-PLC-by-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    25.2
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0397(uM)-Substrate
    G*GTP
    PLC-Ca

    Product
    PLC-Ca-Gq
      Affinity for Gq is > 20 nM (Smrcka et al Science251 804-807 1991) so [Gq].kf = kb so 40nM * 6e5 = kb/kf = 24e3 so kf = 4.2e-5, kb =1



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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