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Molecule Parameter List for E0*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
E0* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1011002

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • MAPK_Asthagiri_
    adapt_fb
  • 45Pathway
    MAPK 
    This is a complex model to examine mechanisms that govern MAPK pathway dynamics in Chinese hamster ovary (CHO) cell lines, particularly the role of adapter targeted negative feedback mechanism in generating complete signal adaptation. This model simulates the results as per the figure 7A of the paper by Asthagiri AR and Lauffenburger DA. Biotechnol Prog. (2001) 17(2):227-39.

    E0* acting as a Molecule in  
    MAPK_Asthagiri_adapt_fb Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    E0*
  • MAPK_Asthagiri_
    adapt_fb

    Accession No. : 45
  • MAPK
    Pathway No. : 193
    0390No
    Active form of enzyme from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

    E0* acting as an Enzyme in  
    MAPK_Asthagiri_adapt_fb Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    E0* /
    E1_activ
  • MAPK_Asthagiri_
    adapt_fb

    Accession No. : 45
  • MAPK
    Pathway No. : 193
    0.06000010.15explicit E-S complexSubstrate
    E1

    Product
    E1*
    k1+ =6*10^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

    E0* acting as a Substrate for an Enzyme in  
    MAPK_Asthagiri_adapt_fb Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    E4*  /
    E4*_adpt_fb
  • MAPK_Asthagiri_
    adapt_fb

    Accession No. : 45
  • MAPK
    Pathway No. : 193
    0.06000010.15explicit E-S complexSubstrate
    E0*

    Product
    A2-
    Implementation of adapter feedback induces adaptation of not only the target signal e0* but also signals downstream of the target. k5+ =6*10^8 M-1 min-1, k5- =30 min-1, kcat,5 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

    E0* acting as a Product in a reaction in  
    MAPK_Asthagiri_adapt_fb Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1C*A1-bind-A2
  • MAPK_Asthagiri_
    adapt_fb

    Accession No. : 45
  • MAPK
    Pathway No. : 193
    5.0001
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.1(uM)-Substrate
    A2
    C*A1

    Product
    E0*
      kf2 and kr2 are 3*10^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
    2C*-bind-A1A2
  • MAPK_Asthagiri_
    adapt_fb

    Accession No. : 45
  • MAPK
    Pathway No. : 193
    5.0001
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.1(uM)-Substrate
    A1A2
    C*

    Product
    E0*
      Kf12 and Kr12 are 3*10^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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