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Molecule Parameter List for PKA-active | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | PKA_2003 | 47 | Network |
Shared_Object_PKA_2003, PKA, AC,
Gs | | This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here. |
PKA-active acting as a Molecule in PKA_2003 Network
PKA-active acting as an Enzyme in PKA_2003 Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | PKA-active /
phosph-PDE
| PKA_2003
Accession No. : 47 | Shared_Object_
PKA_2003
Pathway No. : 195 | 7.5 | 9 | 4 | explicit E-S complex | Substrate
cAMP-PDE
Product
cAMP-PDE*
| | See Bramson et al CRC crit rev Biochem 15:2 93-124. The rates there are for peptide substrates and too fast. Scaled down by a factor of 3 as per Cohen et al FEBS Lett 76:182-86 (1977). |
PKA-active acting as a Substrate in a reaction in PKA_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | inhib-PKA | PKA_2003
Accession No. : 47 | PKA
Pathway No. : 196 | 60
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate
PKA-active
PKA-inhibitor
Product
inhibited-PKA
| | This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though. |
PKA-active acting as a Product in a reaction in PKA_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | Release-C1 | PKA_2003
Accession No. : 47 | PKA
Pathway No. : 196 | 60
(s^-1) | 18
(uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate
cAMP4.R2C2
Product
PKA-active
cAMP4.R2C
| | | This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10 | | 2 | Release-C2 | PKA_2003
Accession No. : 47 | PKA
Pathway No. : 196 | 60
(s^-1) | 18
(uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate
cAMP4.R2C
Product
PKA-active
cAMP4.R2
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| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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