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Molecule Parameter List for Gs.AC

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Gs.AC participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
PKA_200347Network
Shared_Object_PKA_2003 PKA AC 
Gs 
This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here.

Gs.AC acting as a Molecule in  
PKA_2003 Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
Gs.ACPKA_2003
Accession No. : 47
AC
Pathway No. : 197
01000No
This is the active form of the cyclase.

Gs.AC acting as an Enzyme in  
PKA_2003 Network
Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
Gs.AC /
cyclase
PKA_2003
Accession No. : 47
AC
Pathway No. : 197
20184Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate
ATP

Product
cAMP
See Feinstein et al PNAS 88:10173-10177, Jacobowitz et al JBC 286(6):3829-3832 Smigel, JBC 261(4):1976-1982 (1986)

Gs.AC acting as a Product in a reaction in  
PKA_2003 Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
Gs-bind-ACPKA_2003
Accession No. : 47
AC
Pathway No. : 197
499.998
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.002(uM)-Substrate
AC
GTP.Ga

Product
Gs.AC
Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer Jacobowitz thesis (Mount Sinai 1995) data indicates it is more like 2 nM.



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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