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Molecule Parameter List for PKC-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000040

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
PKC_200348Pathway
PKC_2003 PKC 
This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here.

PKC-cytosolic acting as a Molecule in  
PKC_2003 Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
PKC-cytosolicPKC_2003
Accession No. : 48
PKC
Pathway No. : 200
11000No
Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen

PKC-cytosolic acting as a Substrate in a reaction in  
PKC_2003 Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1PKC-act-by-CaPKC_2003
Accession No. : 48
PKC
Pathway No. : 200
0.6
(uM^-1 s^-1)
0.5
(s^-1)
Kd(bf) = 0.8333(uM)-Substrate
Ca
PKC-cytosolic

Product
PKC-Ca
  Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
2PKC-basal-actPKC_2003
Accession No. : 48
PKC
Pathway No. : 200
1
(s^-1)
50
(s^-1)
Keq = 50(uM)0.02secSubstrate
PKC-cytosolic

Product
PKC-basal*
  Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher.
3PKC-act-by-AAPKC_2003
Accession No. : 48
PKC
Pathway No. : 200
0.0001
(uM^-1 s^-1)
0.1
(s^-1)
Kd(bf) = 833.3333(uM)-Substrate
AA
PKC-cytosolic

Product
PKC-AA*
  Raise kf from 1.667e-10 to 2e-10 to get better match to data.
4PKC-n-DAGPKC_2003
Accession No. : 48
PKC
Pathway No. : 200
0.0006
(uM^-1 s^-1)
0.1
(s^-1)
Kd(bf) = 166.6667(uM)-Substrate
DAG
PKC-cytosolic

Product
PKC-DAG
  kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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