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Molecule Parameter List for CaMKII

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CaMKII participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000220

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
CaMKII_200349Network
Shared_Object_CaMKII_2003 CaMKII CaM 
PP1 PP2B 
Model of regulation of CaMKII by Calcium, including parallel excitatory input from CaM and inhibitory input from PP1 as regulated by Calcineurin and PKA. Cell type: neuronal.
Bhalla US. Biophys J. 2004 Aug;87(2):733-44.

CaMKII acting as a Molecule in  
CaMKII_2003 Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
CaMKIICaMKII_2003
Accession No. : 49
CaMKII
Pathway No. : 202
701000No
Huge conc of CaMKII. In PSD it is 20-40% of protein, so we assume it is around 2.5% of protein in spine as a whole. This level is so high it is unlikely to matter much if we are off a bit. This comes to about 70 uM.

CaMKII acting as a Product of an Enzyme in  
CaMKII_2003 Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1PP1-active  /
Deph-thr306
CaMKII_2003
Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 5.099070.354explicit E-S complexSubstrate
    CaMK-thr306

    Product
    CaMKII
        See Cohen et al
    2PP1-active  /
    Deph_thr286b
    CaMKII_2003
    Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 5.099070.354explicit E-S complexSubstrate
    CaMKII-thr286

    Product
    CaMKII

    CaMKII acting as a Substrate in a reaction in  
    CaMKII_2003 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1CaMKII-bind-CaMCaMKII_2003
    Accession No. : 49
    CaMKII
    Pathway No. : 202
    49.9998
    (uM^-1 s^-1)
    5
    (s^-1)
    Kd(bf) = 0.1(uM)-Substrate
    CaM-Ca4
    CaMKII

    Product
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    2basal-activityCaMKII_2003
    Accession No. : 49
    CaMKII
    Pathway No. : 202
    0.003
    (s^-1)
    0
    (s^-1)
    --Substrate
    CaMKII

    Product
    CaMKII-thr286
      This reaction represents one of the big unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented.



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