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Molecule Parameter List for CaMKII-thr286 | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
CaMKII-thr286 acting as a Molecule in CaMKII_2003 Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | CaMKII-thr286 | CaMKII_2003
Accession No. : 49 | CaMKII
Pathway No. : 202 | 0 | 1000 | No | | I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off. |
CaMKII-thr286 acting as a Summed Molecule in CaMKII_2003 Network
CaMKII-thr286 acting as a Substrate for an Enzyme in CaMKII_2003 Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | tot_CaM_CaMKII /
CaM_act_305 | CaMKII_2003
Accession No. : 49 | CaMKII
Pathway No. : 202 | 113.6 | 6 | 4 | explicit E-S complex | Substrate
CaMKII-thr286
Product
CaMKII***
| | | Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5. | | 2 | tot_autonomous_
CaMKII /
auton_305 | CaMKII_2003
Accession No. : 49 | CaMKII
Pathway No. : 202 | 175.003 | 6 | 4 | explicit E-S complex | Substrate
CaMKII-thr286
Product
CaMKII***
| | | See Hanson and Schulman again, for afterburst rates of phosph. | | 3 | PP1-active /
Deph_thr286b | CaMKII_2003
Accession No. : 49 | Shared_Object_
CaMKII_2003
Pathway No. : 201 | 5.09907 | 0.35 | 4 | explicit E-S complex | Substrate
CaMKII-thr286
Product
CaMKII
|
CaMKII-thr286 acting as a Product of an Enzyme in CaMKII_2003 Network
CaMKII-thr286 acting as a Substrate in a reaction in CaMKII_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | CaMK-thr286-bind
-CaM | CaMKII_2003
Accession No. : 49 | CaMKII
Pathway No. : 202 | 1000.2
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate
CaM-Ca4
CaMKII-thr286
Product
CaMKII-thr286*-C
aM
| | Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 |
CaMKII-thr286 acting as a Product in a reaction in CaMKII_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | basal-activity | CaMKII_2003
Accession No. : 49 | CaMKII
Pathway No. : 202 | 0.003
(s^-1) | 0
(s^-1) | - | - | Substrate
CaMKII
Product
CaMKII-thr286
| | This reaction represents one of the big unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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