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Molecule Parameter List for I1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
I1 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001301

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
CaMKII_200349Network
Shared_Object_CaMKII_2003 CaMKII CaM 
PP1 PP2B 
Model of regulation of CaMKII by Calcium, including parallel excitatory input from CaM and inhibitory input from PP1 as regulated by Calcineurin and PKA. Cell type: neuronal.
Bhalla US. Biophys J. 2004 Aug;87(2):733-44.

I1 acting as a Molecule in  
CaMKII_2003 Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
I1CaMKII_2003
Accession No. : 49
PP1
Pathway No. : 204
1.81000No
I1 is a 'mixed' inhibitor, but at high enz concs it looks like a non-compet inhibitor (Foulkes et al Eur J Biochem 132 309-313 9183). We treat it as non-compet, so it just turns the enz off without interacting with the binding site. Cohen et al ann rev bioch refer to results where conc is 1.5 to 1.8 uM. In order to get complete inhib of PP1, which is at 1.8 uM, we need >= 1.8 uM.

I1 acting as a Substrate for an Enzyme in  
CaMKII_2003 Network
Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
PKA-active  /
PKA-phosph-I1
CaMKII_2003
Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 7.594explicit E-S complexSubstrate
    I1

    Product
    I1*
    #s from Bramson et al CRC crit rev Biochem 15:2 93-124. They have a huge list of peptide substrates and I have chosen high-ish rates. These consts give too much PKA activity, so lower Vmax 1/3. Now, k1 = 3e-5, k2 = 36, k3 = 9 (still pretty fast). Also lower Km 1/3 so k1 = 1e-5 Cohen et al FEBS Lett 76:182-86 1977 say rate =30% PKA act on phosphokinase beta.

    I1 acting as a Product of an Enzyme in  
    CaMKII_2003 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1CaM(Ca)n-CaNAB  /
    dephosph_inhib1
    CaMKII_2003
    Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 4.970760.344explicit E-S complexSubstrate
    I1*

    Product
    I1
    2PP2A  /
  • PP2A-dephosph-I1
  • CaMKII_2003
    Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 7.8282864.16667explicit E-S complexSubstrate
    I1*

    Product
    I1
        PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6
    3CaNAB-Ca4  /
  • dephosph_
    inhib1_noCaM
  • CaMKII_2003
    Accession No. : 49
  • Shared_Object_
    CaMKII_2003

    Pathway No. : 201
  • 4.970760.0344explicit E-S complexSubstrate
    I1*

    Product
    I1
        The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034

    I1 acting as a Product in a reaction in  
    CaMKII_2003 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    dissoc-PP1-I1CaMKII_2003
    Accession No. : 49
    PP1
    Pathway No. : 204
    1
    (s^-1)
    0
    (uM^-1 s^-1)
    --Substrate
    PP1-I1

    Product
    I1
    PP1-active
    Let us assume that the equil in this case is very far over to the right. This is probably safe.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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