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Molecule Parameter List for neurogranin

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
neurogranin participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001111

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • MAPK_network_
    2003
  • 50Network
    Shared_Object_MAPK_network_2003 PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC 
    This is a network model of many pathways present at the neuronal synapse. The network has properties of temporal tuning as well as steady-state computational properties. In its default form the network is bistable.Bhalla US Biophys J. 2004 Aug;87(2):745-53

    neurogranin acting as a Molecule in  
    MAPK_network_2003 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    neurogranin
  • MAPK_network_
    2003

    Accession No. : 50
  • CaM
    Pathway No. : 217
    101000No
    Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding ....

    neurogranin acting as a Substrate for an Enzyme in  
    MAPK_network_2003 Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PKC-active  /
  • PKC-phosph-neuro
    granin
  • MAPK_network_
    2003

    Accession No. : 50
  • Shared_Object_
    MAPK_network_
    2003

    Pathway No. : 206
  • 28.62750.584.03448explicit E-S complexSubstrate
    neurogranin

    Product
    neurogranin*
    Rates from Huang et al ABB 305:2 570-580 1993

    neurogranin acting as a Product of an Enzyme in  
    MAPK_network_2003 Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    CaM(Ca)n-CaNAB  /
  • dephosph_
    neurogranin
  • MAPK_network_
    2003

    Accession No. : 50
  • Shared_Object_
    MAPK_network_
    2003

    Pathway No. : 206
  • 10.0120.673.98507explicit E-S complexSubstrate
    neurogranin*

    Product
    neurogranin
    From Seki et al ABB 316(2):673-679

    neurogranin acting as a Substrate in a reaction in  
    MAPK_network_2003 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • neurogranin-bind
    -CaM
  • MAPK_network_
    2003

    Accession No. : 50
  • CaM
    Pathway No. : 217
    0.3
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 3.3333(uM)-Substrate
    CaM
    neurogranin

    Product
    neurogranin-CaM
    Surprisingly, no direct info on rates from neurogranin at this time. These rates are based on GAP-43 binding studies. As GAP-43 and neurogranin share near identity in the CaM/PKC binding regions, and also similarity in phosph and dephosph rates, I am borrowing GAP-43 kinetic info. See Alexander et al JBC 262:13 6108-6113 1987

    neurogranin acting as a Product in a reaction in  
    MAPK_network_2003 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • dephosph-neurogr
    anin
  • MAPK_network_
    2003

    Accession No. : 50
  • CaM
    Pathway No. : 217
    0.005
    (s^-1)
    0
    (s^-1)
    --Substrate
    neurogranin*

    Product
    neurogranin
    This is put in to keep the basal levels of neurogranin* experimentally reasonable. From various papers, specially Ramakers et al JBC 270:23 1995 13892-13898, it looks like the basal level of phosph is between 20 and 40%. I est around 25 % The kf of 0.005 gives around this level at basal PKC activity levels of 0.1 uM active PKC.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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