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Molecule Parameter List for craf-1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
craf-1 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001100

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
EGFR_MAPK58Network
Shared_Object_EGFR_MAPK MAPK Ras 
EGFR Sos 
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here.
Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

craf-1 acting as a Molecule in  
EGFR_MAPK Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
craf-1EGFR_MAPK
Accession No. : 58
MAPK
Pathway No. : 230
0.21000No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil

craf-1 acting as a Substrate for an Enzyme in  
EGFR_MAPK Network
Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
PKC-active  /
PKC-act-raf
EGFR_MAPK
Accession No. : 58
  • Shared_Object_
    EGFR_MAPK

    Pathway No. : 229
  • 66.666744explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
    Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC

    craf-1 acting as a Product of an Enzyme in  
    EGFR_MAPK Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PPhosphatase2A  /
    craf-deph
    EGFR_MAPK
    Accession No. : 58
  • Shared_Object_
    EGFR_MAPK

    Pathway No. : 229
  • 15.656664.16667explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
    See parent PPhosphatase2A for parms



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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