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Molecule Parameter List for MAPKK*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MAPKK* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1021100

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
EGFR_MAPK58Network
Shared_Object_EGFR_MAPK MAPK Ras 
EGFR Sos 
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here.
Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

MAPKK* acting as a Molecule in  
EGFR_MAPK Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
MAPKK*EGFR_MAPK
Accession No. : 58
MAPK
Pathway No. : 230
01000No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992

MAPKK* acting as an Enzyme in  
EGFR_MAPK Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1MAPKK* /
MAPKKtyr
EGFR_MAPK
Accession No. : 58
MAPK
Pathway No. : 230
0.04629630.154explicit E-S complexSubstrate
MAPK

Product
MAPK-tyr
    The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2MAPKK* /
MAPKKthr
EGFR_MAPK
Accession No. : 58
MAPK
Pathway No. : 230
0.04629630.154explicit E-S complexSubstrate
MAPK-tyr

Product
MAPK*
    Rate consts same as for MAPKKtyr.

MAPKK* acting as a Substrate for an Enzyme in  
EGFR_MAPK Network
Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
PPhosphatase2A  /
MAPKK-deph
EGFR_MAPK
Accession No. : 58
  • Shared_Object_
    EGFR_MAPK

    Pathway No. : 229
  • 15.656664.16667explicit E-S complexSubstrate
    MAPKK*

    Product
    MAPKK-ser
    See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

    MAPKK* acting as a Product of an Enzyme in  
    EGFR_MAPK Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    Raf-GTP-Ras*  /
    Raf-GTP-Ras*.2
    EGFR_MAPK
    Accession No. : 58
    MAPK
    Pathway No. : 230
    0.1590910.1054explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
    Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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