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Molecule Parameter List for SHC*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

SHC* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	1	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
EGFR_MAPK	58	Network	Shared_Object_EGFR_MAPK, MAPK, Ras, EGFR, Sos
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here. Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

SHC* acting as a Molecule in EGFR_MAPK Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
SHC*	EGFR_MAPK Accession No. : 58	EGFR Pathway No. : 232	0	1000	No

SHC* acting as a Product of an Enzyme in EGFR_MAPK Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
L.EGFR / phosph_Shc	EGFR_MAPK Accession No. : 58	EGFR Pathway No. : 232	0.833333	0.2	4	explicit E-S complex	Substrate SHC Product SHC*
Rates from Okada et al JBC 270:35 pp 20737 1995 Km = 0.70 to 0.85 uM, Vmax = 4.4 to 5.0 pmol/min. Unfortunately the amount of enzyme is not known, the prep is only partially purified. Time course of phosph is max within 30 sec, falls back within 20 min. Ref: Sasaoka et al JBC 269:51 32621 1994. Use k3 = 0.1 based on this tau.

SHC* acting as a Substrate in a reaction in EGFR_MAPK Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	dephosph_Shc	EGFR_MAPK Accession No. : 58	EGFR Pathway No. : 232	0.0017 (s^-1)	0 (s^-1)	-	-	Substrate SHC* Product SHC
	Time course of decline of phosph is 20 min. Part of this is the turnoff time of the EGFR itself. Lets assume a tau of 10 min for this dephosph. It may be wildly off.
2	Shc_bind_ Sos.Grb2	EGFR_MAPK Accession No. : 58	Sos Pathway No. : 233	0.5 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.2(uM)	-	Substrate SHC* Sos.Grb2 Product Shc*.Sos.Grb2
	Sasaoka et al JBC 269:51 pp 32621 1994, table on pg 32623 indicates that this pathway accounts for about 50% of the GEF activation. (88% - 39%). Error is large, about 20%. Fig 1 is most useful in constraining rates. Chook et al JBC 271:48 pp 30472, 1996 say that the Kd is 0.2 uM for Shc binding to EGFR. The Kd for Grb direct binding is 0.7, so we'll ignore it.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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