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Molecule Parameter List for Grb2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Grb2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000020

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
EGFR_MAPK58Network
Shared_Object_EGFR_MAPK MAPK Ras 
EGFR Sos 
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here.
Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

Grb2 acting as a Molecule in  
EGFR_MAPK Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
Grb2EGFR_MAPK
Accession No. : 58
Sos
Pathway No. : 233
11000No
There is probably a lot of it in the cell: it is also known as Ash (abundant src homology protein I think). Also Waters et al JBC 271:30 18224 1996 say that only a small fraction of cellular Grb is precipitated out when SoS is precipitated. As most of the Sos seems to be associated with Grb2, it would seem like there is a lot of the latter. Say 1 uM. I haven't been able to find a decent....

Grb2 acting as a Substrate in a reaction in  
EGFR_MAPK Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1Grb2_bind_Sos*EGFR_MAPK
Accession No. : 58
Sos
Pathway No. : 233
0.025
(uM^-1 s^-1)
0.0168
(s^-1)
Kd(bf) = 0.672(uM)-Substrate
Grb2
Sos*

Product
Sos*.Grb2
  Same rates as Grb2_bind_Sos: Porfiri and McCormick JBC 271:10 pp 5871 1996 show that the binding is not affected by the phosph.
2Grb2_bind_SosEGFR_MAPK
Accession No. : 58
Sos
Pathway No. : 233
0.025
(uM^-1 s^-1)
0.0168
(s^-1)
Kd(bf) = 0.672(uM)-Substrate
Grb2
Sos

Product
Sos.Grb2
  As there are 2 SH3 domains, this reaction could be 2nd order. I have a Kd of 22 uM from peptide binding (Lemmon et al JBC 269:50 pg 31653). However, Chook et al JBC 271:48 pg30472 say it is 0.4uM with purified proteins, so we believe them. They say it is 1:1 binding.



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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