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Molecule Parameter List for R2C2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*R2C2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
fig4_synapse	3	Network	Shared_Object_fig4_synapse, PKC, PLA2, PLCbeta, Gq, MAPK, Ras, CaMKII, CaM, PP1, PP2B, PKA, AC
This is the composite model of 4 kinases: PKC, MAPK, PKA and CaMKII and numerous regulatory pathways involved in synaptic signaling. From Bhalla US and Iyengar R. Science (1999) 283(5400):381-7.This model comes from figure 4 of that paper. Demonstration script files for generating the figures in the paper, including figure 4, are available here.

R2C2 acting as a Molecule in fig4_synapse Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
R2C2	fig4_synapse Accession No. : 3	PKA Pathway No. : 30	0.5	1000	No
PKA: Protein kinase activated by cAMP. R2C2 complex consists of 2 catalytic (C) subunits, and the R-dimer. There are two types of cAMP binding sites termed A and B according to the rate with which they exchange bound labelled nucleotide. Dagfinn Ogreid and Stein Ove Doskeland (1981) Int.J.Biochem. 13(1):1-19 Physiological concentration of PKA = 0.59 uM (cardiac myocyte) Jeffrey J. Saucerman et al (2003) J.Biol.Chem. 278(48):47997-48003

R2C2 acting as a Substrate in a reaction in fig4_synapse Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
cAMP-bind-site-B 1	fig4_synapse Accession No. : 3	PKA Pathway No. : 30	54 (uM^-1 s^-1)	33 (s^-1)	Kd(bf) = 0.6111(uM)	-	Substrate R2C2 cAMP Product R2C2-cAMP
Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741 Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595 Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7

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