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Molecule Parameter List for Grb2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Grb2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000020

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_PKM_Tuning
  • 76Network
    PKC Shared_Object_Ajay_Bhalla_2004_PKM_tuning PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC PKM 
    This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

    Grb2 acting as a Molecule in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    Grb2
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Sos
    Pathway No. : 320
    11.5No
    There is probably a lot of it in the cell: it is also known as Ash (abundant src homology protein I think). Also Waters et al JBC 271:30 18224 1996 say that only a small fraction of cellular Grb is precipitated out when SoS is precipitated. As most of the Sos seems to be associated with Grb2, it would seem like there is a lot of the latter. Say 1 uM. I haven't been able to find a decent....

    Grb2 acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Grb2_bind_Sos*
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Sos
    Pathway No. : 320
    0.025
    (uM^-1 s^-1)
    0.0168
    (s^-1)
    Kd(bf) = 0.672(uM)-Substrate
    Grb2
    Sos*

    Product
    Sos*.Grb2
      Same rates as Grb2_bind_Sos: Porfiri and McCormick JBC 271:10 pp 5871 1996 show that the binding is not affected by the phosph.
    2Grb2_bind_Sos
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Sos
    Pathway No. : 320
    0.025
    (uM^-1 s^-1)
    0.0168
    (s^-1)
    Kd(bf) = 0.672(uM)-Substrate
    Grb2
    Sos

    Product
    Sos.Grb2
      As there are 2 SH3 domains, this reaction could be 2nd order. I have a Kd of 22 uM from peptide binding (Lemmon et al JBC 269:50 pg 31653). However, Chook et al JBC 271:48 pg30472 say it is 0.4uM with purified proteins, so we believe them. They say it is 1:1 binding.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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