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Molecule Parameter List for AC2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
AC2* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010120

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_PKM_Tuning
  • 76Network
    PKC Shared_Object_Ajay_Bhalla_2004_PKM_tuning PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC PKM 
    This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

    AC2* acting as a Molecule in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    AC2*
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    01.5No
    This version is activated by Gs and by a betagamma and phosphorylation.

    AC2* acting as an Enzyme in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    AC2* /
    kenz
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    20.115374explicit E-S complexSubstrate
    ATP

    Product
    cAMP
    Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18

    AC2* acting as a Product of an Enzyme in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PKC-active  /
    phosph-AC2
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Shared_Object_
    Ajay_Bhalla_
    2004_PKM_tuning

    Pathway No. : 312
  • 33.333244explicit E-S complexSubstrate
    AC2

    Product
    AC2*
    Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4

    AC2* acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1dephosph-AC2
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    0.1
    (s^-1)
    0
    (s^-1)
    --Substrate
    AC2*

    Product
    AC2
      Random rate.
    2Gs-bind-AC2*
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    833.283
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0012(uM)-Substrate
    AC2*
    Gs-alpha

    Product
    AC2*-Gs
      kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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