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Molecule Parameter List for Gs-alpha

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Gs-alpha participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000030

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_PKM_Tuning
  • 76Network
    PKC Shared_Object_Ajay_Bhalla_2004_PKM_tuning PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC PKM 
    This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

    Gs-alpha acting as a Molecule in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    Gs-alpha
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Shared_Object_
    Ajay_Bhalla_
    2004_PKM_tuning

    Pathway No. : 312
  • 01.5No

    Gs-alpha acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Gs-bind-AC2
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    499.995
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.002(uM)-Substrate
    AC2
    Gs-alpha

    Product
    AC2-Gs
      Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer's thesis data indicates it is more like 2 nM. kf = kb/1200 = 8.33e-4
    2Gs-bind-AC1
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    126
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0079(uM)-Substrate
    AC1
    Gs-alpha

    Product
    AC1-Gs
      Half-max 8nM from Tang et al JBC266:13 8595-8603 kb/kf = 8 nM = 4800#/cell so kf = kb * 2.08e-4
    3Gs-bind-AC2*
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • AC
    Pathway No. : 327
    833.283
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0012(uM)-Substrate
    AC2*
    Gs-alpha

    Product
    AC2*-Gs
      kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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