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Molecule Parameter List for PIP2-Ca-PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PIP2-Ca-PLA2* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
  • 78Network
    Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC 
    This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

    PIP2-Ca-PLA2* acting as a Molecule in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PIP2-Ca-PLA2*
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    01.5No

    PIP2-Ca-PLA2* acting as an Enzyme in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PIP2-Ca-PLA2* /
    kenz
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    20364explicit E-S complexSubstrate
    APC

    Product
    AA
    10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.

    PIP2-Ca-PLA2* acting as a Product in a reaction in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • PIP2-Ca-PLA2-act
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    0.012
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 8.3335(uM)-Substrate
    PLA2-Ca*
    temp-PIP2

    Product
    PIP2-Ca-PLA2*



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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