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Molecule Parameter List for SHC*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
SHC* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000120

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
  • 78Network
    Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC 
    This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

    SHC* acting as a Molecule in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    SHC*
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • EGFR
    Pathway No. : 354
    01.5No

    SHC* acting as a Product of an Enzyme in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    L.EGFR  /
    phosph_Shc
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • EGFR
    Pathway No. : 354
    0.8333540.24explicit E-S complexSubstrate
    SHC

    Product
    SHC*
    Rates from Okada et al JBC 270:35 pp 20737 1995 Km = 0.70 to 0.85 uM, Vmax = 4.4 to 5.0 pmol/min. Unfortunately the amount of enzyme is not known, the prep is only partially purified. Time course of phosph is max within 30 sec, falls back within 20 min. Ref: Sasaoka et al JBC 269:51 32621 1994. Use k3 = 0.1 based on this tau.

    SHC* acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1dephosph_Shc
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • EGFR
    Pathway No. : 354
    0.0017
    (s^-1)
    0
    (s^-1)
    --Substrate
    SHC*

    Product
    SHC
      Time course of decline of phosph is 20 min. Part of this is the turnoff time of the EGFR itself. Lets assume a tau of 10 min for this dephosph. It may be wildly off.
    2
  • Shc_bind_
    Sos.Grb2
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • Sos
    Pathway No. : 355
    0.5
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.2(uM)-Substrate
    SHC*
    Sos.Grb2

    Product
    Shc*.Sos.Grb2
      Sasaoka et al JBC 269:51 pp 32621 1994, table on pg 32623 indicates that this pathway accounts for about 50% of the GEF activation. (88% - 39%). Error is large, about 20%. Fig 1 is most useful in constraining rates. Chook et al JBC 271:48 pp 30472, 1996 say that the Kd is 0.2 uM for Shc binding to EGFR. The Kd for Grb direct binding is 0.7, so we'll ignore it.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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