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Molecule Parameter List for PDE1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PDE1 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010010

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
  • 78Network
    Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC 
    This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

    PDE1 acting as a Molecule in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PDE1
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • AC
    Pathway No. : 362
    21.5No
    CaM-Dependent PDE. Amount calculated from total rate in brain vs. specific rate.

    PDE1 acting as an Enzyme in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PDE1 /
    PDE1
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • AC
    Pathway No. : 362
    39.70061.6674.0012explicit E-S complexSubstrate
    cAMP

    Product
    AMP
    Rate is 1/6 of the CaM stim form. We'll just reduce all lf k1, k2, k3 so that the Vmax goes down 1/6.

    PDE1 acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    CaM_bind_PDE1
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • AC
    Pathway No. : 362
    720
    (uM^-1 s^-1)
    5
    (s^-1)
    Kd(bf) = 0.0069(uM)-Substrate
    CaM-Ca4
    PDE1

    Product
    CaM.PDE1
    For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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