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Molecule Parameter List for PKC-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000040

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_Bistable
  • 79Network
    Shared_Object_Ajay_Bhalla_2007_Bistable PKC PLA2 
    MAPK Ras CaM 
    This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII.
    The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

    PKC-cytosolic acting as a Molecule in  
    Ajay_Bhalla_2007_Bistable Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PKC-cytosolic
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    1125.7No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen

    PKC-cytosolic acting as a Substrate in a reaction in  
    Ajay_Bhalla_2007_Bistable Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-Ca
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    2PKC-basal-act
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    1
    (s^-1)
    50
    (s^-1)
    Keq = 50(uM)0.02secSubstrate
    PKC-cytosolic

    Product
    PKC-basal*
      Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher.
    3PKC-act-by-AA
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    0.0001
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 833.3264(uM)-Substrate
    AA
    PKC-cytosolic

    Product
    PKC-AA*
      Raise kf from 1.667e-10 to 2e-10 to get better match to data.
    4PKC-n-DAG
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6656(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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