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Molecule Parameter List for PIP2-PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

PIP2-PLA2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_Bistable	79	Network	Shared_Object_Ajay_Bhalla_2007_Bistable, PKC, PLA2, MAPK, Ras, CaM
This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII. The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

PIP2-PLA2* acting as a Molecule in Ajay_Bhalla_2007_Bistable Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PIP2-PLA2*	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	0	125.7	No

PIP2-PLA2* acting as an Enzyme in Ajay_Bhalla_2007_Bistable Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PIP2-PLA2* / kenz	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	20	11.04	4	explicit E-S complex	Substrate APC Product AA
10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme

PIP2-PLA2* acting as a Product in a reaction in Ajay_Bhalla_2007_Bistable Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PIP2-PLA2-act	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6632(uM)	-	Substrate PLA2-cytosolic temp-PIP2 Product PIP2-PLA2*

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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