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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
DAG participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000030

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_Bistable
  • 79Network
    Shared_Object_Ajay_Bhalla_2007_Bistable PKC PLA2 
    MAPK Ras CaM 
    This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII.
    The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

    DAG acting as a Molecule in  
    Ajay_Bhalla_2007_Bistable Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    DAG
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • Shared_Object_
    Ajay_Bhalla_
    2007_Bistable

    Pathway No. : 363
  • 12.1999125.7Yes

    DAG acting as a Substrate in a reaction in  
    Ajay_Bhalla_2007_Bistable Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    0.008
    (uM^-1 s^-1)
    8.6348
    (s^-1)
    Kd(bf) = 1079.377(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    2PKC-n-DAG
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PKC
    Pathway No. : 364
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6656(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    3DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_Bistable

    Accession No. : 79
  • PLA2
    Pathway No. : 365
    0.003
    (uM^-1 s^-1)
    4
    (s^-1)
    Kd(bf) = 1333.3422(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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