NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Molecule Parameter List for PKC-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences2500007424

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12
  • 81NetworkShared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12 
    PKC MAPK Ras CaM PKM chain kinetics PKC MAPK Ras CaM PKM kinetics[1] 
    PKC MAPK Ras CaM PKM kinetics[2] PKC MAPK Ras CaM PKM kinetics[3] 
    PKC MAPK MAPK Ras CaM PKM kinetics[4] PKC MAPK Ras CaM PKM 
    kinetics[5] PKC MAPK Ras kinetics[6] CaM PKM PKC MAPK Ras 
    CaM PKM kinetics[7] PKC Ras CaM PKM kinetics[8] PKC MAPK 
    Ras CaM PKM kinetics[9] PKC MAPK Ras CaM PKM kinetics[10] 
    PKC MAPK Ras CaM PKM kinetics[11] PKC MAPK Ras CaM PKM kinetics[12] 
    PKC MAPK Ras CaM PKM kinetics[13] PKC MAPK Ras CaM PKM kinetics[14] 
    PKC MAPK Ras CaM PKM kinetics[15] PKC MAPK Ras CaM PKM kinetics[16] 
    PKC MAPK Ras CaM PKM kinetics[17] PKC MAPK Ras CaM PKM kinetics[18] 
    PKC MAPK Ras CaM PKM kinetics[19] PKC MAPK Ras CaM PKM kinetics[20] 
    PKC MAPK Ras CaM PKM kinetics[21] PKC MAPK Ras CaM PKM kinetics[22] 
    PKC MAPK Ras CaM PKM kinetics[23] PKC MAPK Ras CaM PKM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec.
    The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper.
    This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper

    PKC-cytosolic acting as a Molecule in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 376
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 383
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 389
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 395
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 401
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 408
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 414
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 420
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 426
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 431
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 437
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 443
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 449
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 455
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 461
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 467
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 473
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 479
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 485
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 491
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 497
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 503
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 509
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 515
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen
    PKC-cytosolic
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 521
    11.5No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen

    PKC-cytosolic acting as a Substrate in a reaction in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 376
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    2PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 376
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    3diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    4PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 383
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    5PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 383
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    6diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    7PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 401
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    8PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 389
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    9PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 389
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    10diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    11PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 395
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    12PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 395
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    13diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    14PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 426
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    15PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 401
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    16diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    17PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 408
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    18PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 408
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    19diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    20PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 414
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    21PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 414
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    22diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    23PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 420
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    24PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 420
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    25diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    26PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 473
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    27PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 473
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    28PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 426
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    29diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    30PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 431
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    31PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 431
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    32diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    33PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 437
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    34PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 437
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    35diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    36PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 443
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    37PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 443
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    38diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    39PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 449
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    40PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 449
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    41diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    42PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 455
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    43PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 455
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    44diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    45PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 461
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    46PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 461
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    47diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    48PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 467
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    49PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 467
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    50diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    51diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    52PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 479
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    53PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 479
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    54diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    55PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 485
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    56PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 485
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    57diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    58PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 491
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    59PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 491
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    60diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    61PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 497
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    62PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 497
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    63diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    64PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 503
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    65PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 503
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    66diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    67PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 509
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    68PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 509
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    69diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    70PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 515
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    71PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 515
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    72diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    73PKC-act-by-Ca
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 521
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    74PKC-n-DAG
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • PKC
    Pathway No. : 521
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9

    PKC-cytosolic acting as a Product in a reaction in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    2diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    3diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    4diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    5diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    6diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    7diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    8diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    9diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    10diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    11diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    12diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    13diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    14diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    15diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    16diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    17diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    18diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    19diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    20diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    21diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    22diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    23diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic
    24diff
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0.01
    (s^-1)
    0.01
    (s^-1)
    Keq = 1(uM)50secSubstrate
    PKC-cytosolic

    Product
    PKC-cytosolic



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
    This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.