DOQCS database

NCBS Home page

Accession List

Pathway List

Authorized Users

Help

News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.

Molecule Parameter List for Raf-GTP-Ras*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

**Raf-GTP-Ras*** participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	50	0	0	0	25

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_bhalla_ 2007_ReacDiff1_ 1e-12	81	Network	Shared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, kinetics[3], PKC, MAPK, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, kinetics[6], CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec. The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper. This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper

Raf*-GTP-Ras acting as a Molecule in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0	1.5	No
Raf-GTP-Ras*	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0	1.5	No

Raf*-GTP-Ras acting as an Enzyme in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
2	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
3	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
4	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
5	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
6	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
7	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
8	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
9	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
10	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
11	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
12	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
13	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
14	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
15	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
16	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
17	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
18	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
19	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
20	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
21	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
22	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
23	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
24	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
25	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
26	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
27	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
28	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
29	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
30	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
31	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
32	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
33	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
34	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
35	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
36	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
37	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
38	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
39	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
40	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
41	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
42	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
43	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
44	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
45	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
46	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
47	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
48	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
49	Raf-GTP-Ras* / Raf*-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
50	Raf-GTP-Ras* / Raf*-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6

Raf*-GTP-Ras acting as a Product in a reaction in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
2	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
3	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
4	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
5	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
6	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
7	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
8	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
9	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
10	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
11	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
12	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
13	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
14	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
15	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
16	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
17	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
18	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
19	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
20	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
21	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
22	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
23	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
24	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
25	Ras-act-craf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.

Enter a Search String

Molecule Parameter List for Raf*-GTP-Ras

Molecule Parameter List for Raf-GTP-Ras*