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Molecule Parameter List for GTP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
GTP-Ras participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences25002575750

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12
  • 81NetworkShared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12 
    PKC MAPK Ras CaM PKM chain kinetics PKC MAPK Ras CaM PKM kinetics[1] 
    PKC MAPK Ras CaM PKM kinetics[2] PKC MAPK Ras CaM PKM kinetics[3] 
    PKC MAPK MAPK Ras CaM PKM kinetics[4] PKC MAPK Ras CaM PKM 
    kinetics[5] PKC MAPK Ras kinetics[6] CaM PKM PKC MAPK Ras 
    CaM PKM kinetics[7] PKC Ras CaM PKM kinetics[8] PKC MAPK 
    Ras CaM PKM kinetics[9] PKC MAPK Ras CaM PKM kinetics[10] 
    PKC MAPK Ras CaM PKM kinetics[11] PKC MAPK Ras CaM PKM kinetics[12] 
    PKC MAPK Ras CaM PKM kinetics[13] PKC MAPK Ras CaM PKM kinetics[14] 
    PKC MAPK Ras CaM PKM kinetics[15] PKC MAPK Ras CaM PKM kinetics[16] 
    PKC MAPK Ras CaM PKM kinetics[17] PKC MAPK Ras CaM PKM kinetics[18] 
    PKC MAPK Ras CaM PKM kinetics[19] PKC MAPK Ras CaM PKM kinetics[20] 
    PKC MAPK Ras CaM PKM kinetics[21] PKC MAPK Ras CaM PKM kinetics[22] 
    PKC MAPK Ras CaM PKM kinetics[23] PKC MAPK Ras CaM PKM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec.
    The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper.
    This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper

    GTP-Ras acting as a Molecule in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
    GTP-Ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    01.5No
    Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437

    GTP-Ras acting as a Substrate for an Enzyme in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    2GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    3GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    4GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    5GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    6GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    7GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    8GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    9GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    10GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    11GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    12GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    13GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    14GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    15GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    16GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    17GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    18GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    19GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    20GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    21GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    22GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    23GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    24GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    25GAP  /
    GAP-inact-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.

    GTP-Ras acting as a Product of an Enzyme in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    2GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    3CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    4inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    5GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    6CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    7inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    8GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    9CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    10inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    11GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    12CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    13inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    14GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    15CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    16inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    17GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    18CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    19inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    20GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    21CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    22inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    23GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    24CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    25inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    26GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    27CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    28inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    29GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    30CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    31inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    32GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    33CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    34inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    35GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    36CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    37inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    38GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    39CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    40inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    41GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    42CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    43inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    44GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    45CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    46inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    47GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    48CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    49inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    50GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    51CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    52inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    53GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    54CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    55inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    56GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    57CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    58inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    59GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    60CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    61inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    62GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    63CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    64inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    65GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    66CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    67inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    68GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    69CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    70inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    71GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    72CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    73inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    74GEF*  /
    GEF*-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    75CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.

    GTP-Ras acting as a Substrate in a reaction in  
    Ajay_bhalla_2007_ReacDiff1_1e-12 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    2
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 378
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    3
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Shared_Object_
    Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Pathway No. : 375
  • 0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    4Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics
    Pathway No. : 382
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    5
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 385
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    6
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics
    Pathway No. : 382
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    7Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[1]
    Pathway No. : 388
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    8
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 391
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    9
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[1]
    Pathway No. : 388
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    10Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[2]
    Pathway No. : 394
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    11
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 397
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    12
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[2]
    Pathway No. : 394
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    13Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[3]
    Pathway No. : 400
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    14
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 404
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    15
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[3]
    Pathway No. : 400
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    16Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[4]
    Pathway No. : 407
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    17
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 410
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    18
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[4]
    Pathway No. : 407
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    19Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[5]
    Pathway No. : 413
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    20
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 416
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    21
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[5]
    Pathway No. : 413
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    22Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[6]
    Pathway No. : 417
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    23
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 422
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    24
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[6]
    Pathway No. : 417
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    25Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[7]
    Pathway No. : 425
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    26
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 427
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    27
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[7]
    Pathway No. : 425
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    28Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[8]
    Pathway No. : 430
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    29
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 433
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    30
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[8]
    Pathway No. : 430
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    31Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[9]
    Pathway No. : 436
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    32
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 439
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    33
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[9]
    Pathway No. : 436
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    34Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[10]
    Pathway No. : 442
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    35
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 445
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    36
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[10]
    Pathway No. : 442
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    37Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[11]
    Pathway No. : 448
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    38
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 451
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    39
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[11]
    Pathway No. : 448
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    40Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[12]
    Pathway No. : 454
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    41
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 457
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    42
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[12]
    Pathway No. : 454
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    43Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[13]
    Pathway No. : 460
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    44
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 463
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    45
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[13]
    Pathway No. : 460
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    46Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[14]
    Pathway No. : 466
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    47
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 469
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    48
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[14]
    Pathway No. : 466
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    49Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[15]
    Pathway No. : 472
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    50
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 475
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    51
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[15]
    Pathway No. : 472
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    52Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[16]
    Pathway No. : 478
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    53
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 481
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    54
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[16]
    Pathway No. : 478
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    55Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[17]
    Pathway No. : 484
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    56
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 487
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    57
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[17]
    Pathway No. : 484
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    58Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[18]
    Pathway No. : 490
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    59
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 493
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    60
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[18]
    Pathway No. : 490
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    61Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[19]
    Pathway No. : 496
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    62
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 499
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    63
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[19]
    Pathway No. : 496
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    64Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[20]
    Pathway No. : 502
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    65
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 505
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    66
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[20]
    Pathway No. : 502
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    67Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[21]
    Pathway No. : 508
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    68
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 511
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    69
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[21]
    Pathway No. : 508
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    70Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[22]
    Pathway No. : 514
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    71
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 517
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    72
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[22]
    Pathway No. : 514
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    73Ras-act-craf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[23]
    Pathway No. : 520
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    74
  • Ras-intrinsic-GT
    Pase
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • Ras
    Pathway No. : 523
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    75
  • Ras-act-unphosph
    -raf
  • Ajay_bhalla_
    2007_ReacDiff1_
    1e-12

    Accession No. : 81
  • kinetics[23]
    Pathway No. : 520
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.



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