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Molecule Parameter List for MAPK*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MAPK* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1021100

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
3d_fold_model8Network
Shared_Object_3d_fold_model PKC MAPK 
PLA2 Ras 
This model is an annotated version of the synaptic signaling network.
The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

MAPK* acting as a Molecule in  
3d_fold_model Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
MAPK*3d_fold_model
Accession No. : 8
  • Shared_Object_
    3d_fold_model

    Pathway No. : 54
  • 01000No
    This version is phosphorylated on both the tyr and thr residues and is active: refs The rate consts are very hard to nail down. Combine Sanghera et al JBC 265(1) :52-57 with Nemenoff et al JBC 93 pp 1960 to get k3=10/sec = k2 (from Nemenoff Vmax) and k1 = (k2 + k3)/Km = 1.3e-6 Or: k3 = 10, k2 = 40, k1 = 3.25e-6

    MAPK* acting as an Enzyme in  
    3d_fold_model Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1MAPK* /
    MAPK*-feedback
    3d_fold_model
    Accession No. : 8
  • Shared_Object_
    3d_fold_model

    Pathway No. : 54
  • 25.641104explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1**
        Ueki et al JBC 269(22):15756-15761 show the presence of this step, but not the rate consts, which are derived from Sanghera et al JBC 265(1):52-57, 1990, see the deriv in the MAPK* notes.
    2MAPK* /
    MAPK*
    3d_fold_model
    Accession No. : 8
  • Shared_Object_
    3d_fold_model

    Pathway No. : 54
  • 25.641204explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

    MAPK* acting as a Substrate for an Enzyme in  
    3d_fold_model Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MKP-1  /
    MKP1-thr-deph
    3d_fold_model
    Accession No. : 8
  • Shared_Object_
    3d_fold_model

    Pathway No. : 54
  • 0.066666714explicit E-S complexSubstrate
    MAPK*

    Product
    MAPK-tyr
    See MKP1-tyr-deph

    MAPK* acting as a Product of an Enzyme in  
    3d_fold_model Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPKK*  /
    MAPKKthr
    3d_fold_model
    Accession No. : 8
    MAPK
    Pathway No. : 56
    0.04629630.154explicit E-S complexSubstrate
    MAPK-tyr

    Product
    MAPK*
    Rate consts same as for MAPKKtyr.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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