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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
DAG participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000030

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
3d_fold_model8Network
Shared_Object_3d_fold_model PKC MAPK 
PLA2 Ras 
This model is an annotated version of the synaptic signaling network.
The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

DAG acting as a Molecule in  
3d_fold_model Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
DAG3d_fold_model
Accession No. : 8
  • Shared_Object_
    3d_fold_model

    Pathway No. : 54
  • 11.6611000Yes
    Baseline is 11.661 from running combined model to steady state. See ALL/NOTES for 23 Apr 1998.

    DAG acting as a Substrate in a reaction in  
    3d_fold_model Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-DAG3d_fold_model
    Accession No. : 8
    PKC
    Pathway No. : 55
    0.008
    (uM^-1 s^-1)
    8.6348
    (s^-1)
    Kd(bf) = 1079.377(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    2PKC-n-DAG3d_fold_model
    Accession No. : 8
    PKC
    Pathway No. : 55
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6667(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    3DAG-Ca-PLA2-act3d_fold_model
    Accession No. : 8
    PLA2
    Pathway No. : 57
    0.003
    (uM^-1 s^-1)
    4
    (s^-1)
    Kd(bf) = 1333.3333(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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