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Molecule Parameter List for PKC-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000040

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • MAPK_MKP1_
    oscillation
  • 9Network
    Shared_Object_MAPK_MKP1_oscillation PKC MAPK 
    PLA2 Ras 
    This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

    PKC-cytosolic acting as a Molecule in  
    MAPK_MKP1_oscillation Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PKC-cytosolic
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PKC
    Pathway No. : 60
    11000No
    Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen

    PKC-cytosolic acting as a Substrate in a reaction in  
    MAPK_MKP1_oscillation Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-Ca
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PKC
    Pathway No. : 60
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
      Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
    2PKC-basal-act
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PKC
    Pathway No. : 60
    1
    (s^-1)
    50
    (s^-1)
    Keq = 50(uM)0.02secSubstrate
    PKC-cytosolic

    Product
    PKC-basal*
      Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher.
    3PKC-act-by-AA
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PKC
    Pathway No. : 60
    0.0001
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 833.3333(uM)-Substrate
    AA
    PKC-cytosolic

    Product
    PKC-AA*
      Raise kf from 1.667e-10 to 2e-10 to get better match to data.
    4PKC-n-DAG
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PKC
    Pathway No. : 60
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6667(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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