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Molecule Parameter List for MKP-1-gene

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MKP-1-gene* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_MKP1_ oscillation	9	Network	Shared_Object_MAPK_MKP1_oscillation, PKC, MAPK, PLA2, Ras
This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

MKP-1-gene acting as a Molecule in MAPK_MKP1_oscillation Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MKP-1-gene	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	1	1000	Yes
3 Feb 2000. This represents the formation of MKP-1 when MAPK is stimulated. Concentration is pretty irrelevant here, set to 1 uM for convenience.

MKP-1-gene acting as a Substrate for an Enzyme in MAPK_MKP1_oscillation Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
nuc_MAPK* / MKP-1-synthesis	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	27.3632	0.0001	10	explicit E-S complex	Substrate MKP-1-gene Product MKP-1
5 Feb 2000 This is a crude model of activation of MKP1 production by MAPK. Time-course poorly constrained: 10 min to > 100 min. Rates are further constrained by the known degradation rates of MKP-1, also in this model. Peak MKP-1 is about 10 times basal (from Brondello et al Science 286:2514 1999). Max MAPK ~.25 uM, degradation 45 min. So this enz has to provide 22nM MKP every 45 min.

MKP-1-gene acting as a Substrate in a reaction in MAPK_MKP1_oscillation Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
MKP-1-basal-synt h	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0 (s^-1)	0 (s^-1)	-	-	Substrate MKP-1-gene Product MKP-1
3 Feb 2000. The levels of basal MAPK are so low that we need to have a basal mechanism for making MKP-1. This is easy, as the degradation tau is 45 min and basal MKP-1 levels are 2.4 nM in our model. x * 1 uM = 2.4e-3 * 0.37e-3 = 8.9e-7 May 30 2k. Basal levels shifted up a bit. kf now 1.0e-6 Jun 3 2k: kf now 8e-7 Jun 4 2k: kf = 7e-7

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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