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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
DAG participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000301

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
PLCbeta113Pathway
PLCbeta 
This is a model of PLCbeta1 in the bovine brain membranes as stimulated by Ca, Gq and PA.
The primary data sources are Irene Litosch Biochemistry (2000) 39(26):7736-43,
Sternweis PC et al. Philos Trans R Soc Lond B Biol Sci. (1992) 336(1276):35-41 and
Smrcka AV et al. Science (1991) 251(4995):804-7.

DAG acting as a Molecule in  
PLCbeta1 Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
DAGPLCbeta1
Accession No. : 13
PLCbeta
Pathway No. : 67
00.0016667No
6 Nov 2001 DAG: diacylglycerol.

DAG acting as a Product of an Enzyme in  
PLCbeta1 Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1Ca.PLCbeta  /
Ca.PLCbeta
PLCbeta1
Accession No. : 13
PLCbeta
Pathway No. : 67
1074Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate
PIP2

Product
DAG
IP3
    3 Nov 2001 Sternweis et al Phil Trans Soc R Lond B Biol Sci 1992 Apr 29; 336(1276);35-41;discussion 41-2. The Km and Vmax values according to Sternweis et al. The Km is fixed to 10uM since there is absence of Gq and so is the Vmax values which is fixed at 7uM since the Km obtained varies between 5 and 20uM and Vmax between 7 and 23umol/min/mg depending on the presence and absence of Gq.
2PA.Ca.PLCbeta  /
PA.Ca.PLCbeta
PLCbeta1
Accession No. : 13
PLCbeta
Pathway No. : 67
10384Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate
PIP2

Product
DAG
IP3
    3 Nov 2001 The Km and Vmax were fixed relatively to the Km and Vmax values of Ca.PLCbeta and Gq.Ca.PLCbeta which were obtained from the works of Sternweis et al and Smrcka et al where the Km varied between 5 and 20uM and Vmax between 7 and 23 umol/min/mg depending on the presence and absence of Gq.
3Gq.Ca.PLCbeta  /
Gq.Ca.PLCbeta
PLCbeta1
Accession No. : 13
PLCbeta
Pathway No. : 67
10234explicit E-S complexSubstrate
PIP2

Product
DAG
IP3
    3 Nov 2001 The Km and Vmax values are fixed accordingly from Sternweis et al Phil Trans R Soc Lond B Bio Sci 1992 Apr 29;336(1276):35-41;discussion 41-2 and Smrcka et al Science 251 804-807 1991 where Km varied between 5 and 20uM and Vmax between 7 and 23 umol/min/mg depending on the presenece and absence of Gq.

DAG acting as a Product in a reaction in  
PLCbeta1 Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
BasalPLCbeta1
Accession No. : 13
PLCbeta
Pathway No. : 67
0.009
(s^-1)
0
(uM^-1 s^-1)
--Substrate
PIP2

Product
DAG
IP3
6 Nov 2001 The basal reaction rates are fixed according to the respomse curves obtained from I. Litosch, Biochem.,2000, 39, 7736-7743.



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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