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Molecule Parameter List for Gq.Ca.PLCbeta

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*Gq.Ca.PLCbeta* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
PLCbeta1	13	Pathway	PLCbeta
This is a model of PLCbeta1 in the bovine brain membranes as stimulated by Ca, Gq and PA. The primary data sources are Irene Litosch Biochemistry (2000) 39(26):7736-43, Sternweis PC et al. Philos Trans R Soc Lond B Biol Sci. (1992) 336(1276):35-41 and Smrcka AV et al. Science (1991) 251(4995):804-7.

Gq.Ca.PLCbeta acting as a Molecule in PLCbeta1 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Gq.Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	0	0.0016667	No
6 Nov 2001 This active enzyme is complex formed by binding of Gq with Ca.PLCbeta where concentration formed is 0 because of the absence of Gq.The response curves are obtained in the absence of Gq by I.Litosch, Biochem. 2000, 39,7736-7743.

Gq.Ca.PLCbeta acting as an Enzyme in PLCbeta1 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Gq.Ca.PLCbeta / Gq.Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	10	23	4	explicit E-S complex	Substrate PIP2 Product DAG IP3
3 Nov 2001 The Km and Vmax values are fixed accordingly from Sternweis et al Phil Trans R Soc Lond B Bio Sci 1992 Apr 29;336(1276):35-41;discussion 41-2 and Smrcka et al Science 251 804-807 1991 where Km varied between 5 and 20uM and Vmax between 7 and 23 umol/min/mg depending on the presenece and absence of Gq.

Gq.Ca.PLCbeta acting as a Product in a reaction in PLCbeta1 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Gq-bind-Ca.PLCbe ta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	50 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.02(uM)	-	Substrate Ca.PLCbeta Gq Product Gq.Ca.PLCbeta
6 Nov 2001 The Kf and Kb values were fixed according to the response curves obtained from I. Litosch, Biochem., 2000, 39, 7736-7743.

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