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Molecule Parameter List for PLA2-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLA2-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001021

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Synaptic_
    Network
  • 16Network
    Shared_Object_Synaptic_Network PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC CaRegulation 
    This model is an annotated version of the synaptic signaling network.
    The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated.
    Bhalla US Biophys J. 2002 Aug;83(2):740-52
    Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

    PLA2-cytosolic acting as a Molecule in  
    Synaptic_Network Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLA2-cytosolic
  • Synaptic_
    Network

    Accession No. : 16
  • PLA2
    Pathway No. : 72
    0.41000No
    cPLA2 IV form has mol wt of 85 Kd. Glaser et al 1993 TIPS 14:92-98. Calculated cytosolic concentration is ~300 nM from Wijkander and Sundler 1991 Eur J Biochem 202:873 Leslie and Channon 1990 BBA 1045:261 use about 400 nM. Decent match. Use 400 nM.

    PLA2-cytosolic acting as a Substrate for an Enzyme in  
    Synaptic_Network Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPK*  /
    MAPK*
  • Synaptic_
    Network

    Accession No. : 16
  • Shared_Object_
    Synaptic_
    Network

    Pathway No. : 70
  • 25.641204explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
    Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz. Numbers are from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al 1993 JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg total protein in fig 5 I take the Vmax to be higher for PLA2 given the fold activation of PLA2 by MAPK. This is actually a balance term between MAPK and the dephosphorylation step.

    PLA2-cytosolic acting as a Substrate in a reaction in  
    Synaptic_Network Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PLA2-Ca-act
  • Synaptic_
    Network

    Accession No. : 16
  • PLA2
    Pathway No. : 72
    1
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.1(uM)-Substrate
    Ca
    PLA2-cytosolic

    Product
    PLA2-Ca*
      Direct activation of PLA2 by Ca. From Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.
    2PIP2-PLA2-act
  • Synaptic_
    Network

    Accession No. : 16
  • PLA2
    Pathway No. : 72
    0.0012
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 416.6667(uM)-Substrate
    PLA2-cytosolic
    temp-PIP2

    Product
    PIP2-PLA2*
      Activation of PLA2 by PIP2. From Leslie and Channon 1990 BBA 1045:261 the stimulation of PLA2 activity by high PIP2 is 7x. In this model we don't really expect any PIP2 stimulus.

    PLA2-cytosolic acting as a Product in a reaction in  
    Synaptic_Network Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • dephosphorylate-
    PLA2*
  • Synaptic_
    Network

    Accession No. : 16
  • PLA2
    Pathway No. : 72
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    Dephosphorylation reaction to balance MAPK phosphorylation of PLA2. This is probably mediated by PP2A. Rates determined to keep the balance of phosphorylated and non-phosphorylated PLA2 reasonable. The constraining factor is the fold activation of PLA2 by MAPK.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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