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Molecule Parameter List for MAPK

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MAPK participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001100

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Synaptic_
    Network
  • 16Network
    Shared_Object_Synaptic_Network PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC CaRegulation 
    This model is an annotated version of the synaptic signaling network.
    The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated.
    Bhalla US Biophys J. 2002 Aug;83(2):740-52
    Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

    MAPK acting as a Molecule in  
    Synaptic_Network Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    MAPK
  • Synaptic_
    Network

    Accession No. : 16
  • MAPK
    Pathway No. : 75
    0.361000No
    Mol wt is 42 KDa. conc is from Sanghera et al JBC 265 pp 52 (1990) They estimate MAPK is 1e-4x total protein, and protein is 15% of cell wt, so MAPK is 1.5e-5g/ml = 0.36uM. Lets use this. Note though that Huang and Ferrell 1996 PNAS 93(19):10078 report 1.2 uM in oocytes. Also note that brain concs may be high. Ortiz et al 1995 J. Neurosci 15(2):1285-1297 report 0.3 ng/ug protein in Cingulate Gyrus and 1.2 ng/ug protein in nucleus accumbens. In hippocampus 270 ng/mg protein for ERK1 and 820 ng/mg protein for ERK 2. If 15% of cell weight is protein, that means that about 300 * 0.15 ng/ul is ERK 1. ie, 45e-9g/1e-6 litre = 45 mg/litre or about 1 uM. For non-neuronal tissues a lower value may be better.

    MAPK acting as a Substrate for an Enzyme in  
    Synaptic_Network Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPKK*  /
    MAPKKtyr
  • Synaptic_
    Network

    Accession No. : 16
  • MAPK
    Pathway No. : 75
    0.04629630.154explicit E-S complexSubstrate
    MAPK

    Product
    MAPK-tyr
    The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, ratio of 4 to get k2=0.6. k1=0.75/46.6nM=2.7e-5 In terms of Michaelis-Menten rates, Km = 0.046, Vmax = 0.15, ratio = 4.

    MAPK acting as a Product of an Enzyme in  
    Synaptic_Network Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MKP-1  /
    MKP1-tyr-deph
  • Synaptic_
    Network

    Accession No. : 16
  • Shared_Object_
    Synaptic_
    Network

    Pathway No. : 70
  • 0.066666714explicit E-S complexSubstrate
    MAPK-tyr

    Product
    MAPK
    The original kinetics from Bhalla and Iyengar Science 1999 have now been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. The main constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. See Charles et al 1993 PNAS 90:5292-5296 and Charles et al Oncogene 7:187-190 Effective Km : 67 nM kcat = 1.43 umol/min/mg



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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