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Molecule Parameter List for GTP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*GTP-Ras* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	4	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
fig2_egfr	1	Network	Shared_Object_fig2_egfr, PKC, PLA2, PLCbeta, Gq, MAPK, Ras, EGFR, Sos, PLC_g, CaRegulation
This network was used to generate figure 2 in Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It consists of the MAPK cascade in a feedback loop with PKC, and receives input from the EGFR in the synapse. Demonstration script files for generating the figures in the paper, including figure 2, are available here.

GTP-Ras acting as a Molecule in fig2_egfr Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
GTP-Ras	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	0	1000	No

GTP-Ras acting as a Substrate for an Enzyme in fig2_egfr Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
GAP / GAP-inact-ras	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	1.0104	10	100	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras

GTP-Ras acting as a Product of an Enzyme in fig2_egfr Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	GEF-Gprot-bg / GEF-bg_act-ras	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	0.505051	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
2	GEF* / GEF*-act-ras	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	0.505051	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
3	CaM-GEF / CaM-GEF-act-ras	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	0.505051	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
4	Shc*.Sos.Grb2 / Sos.Ras_GEF	fig2_egfr Accession No. : 1	Shared_Object_ fig2_egfr Pathway No. : 1	0.505051	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

GTP-Ras acting as a Substrate in a reaction in fig2_egfr Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Ras-act-craf	fig2_egfr Accession No. : 1	Shared_Object_ fig2_egfr Pathway No. : 1	24 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.0208(uM)	-	Substrate GTP-Ras craf-1* Product Raf-GTP-Ras*
2	Ras-intrinsic-GT Pase	fig2_egfr Accession No. : 1	Ras Pathway No. : 7	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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