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Molecule Parameter List for PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLA2* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences25000257424

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff3
  • 84NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff3 PKC PLA2 
    MAPK PLA2 Ras CaM chain kinetics PKC MAPK Ras CaM kinetics[1] 
    PKC PLA2 MAPK Ras CaM kinetics[2] PKC PLA2 MAPK Ras CaM kinetics[3] 
    PKC PLA2 MAPK Ras CaM kinetics[4] PKC PLA2 MAPK Ras CaM kinetics[5] 
    PKC PLA2 MAPK Ras MAPK CaM kinetics[6] PKC PLA2 MAPK Ras 
    CaM kinetics[7] PKC PLA2 MAPK Ras CaM PKC kinetics[8] PLA2 
    MAPK Ras CaM kinetics[9] PKC PLA2 MAPK Ras CaM kinetics[10] 
    PKC PLA2 MAPK Ras CaM kinetics[11] PKC PLA2 MAPK Ras CaM 
    kinetics[12] PKC PLA2 Ras CaM kinetics[13] PKC PLA2 MAPK 
    Ras CaM kinetics[14] PKC PLA2 MAPK Ras CaM kinetics[15] 
    PKC PLA2 MAPK Ras kinetics[16] CaM PKC PLA2 MAPK Ras CaM 
    kinetics[17] PKC PLA2 MAPK Ras CaM kinetics[18] PKC PLA2 
    MAPK Ras CaM kinetics[19] PKC PLA2 MAPK Ras CaM kinetics[20] 
    PKC PLA2 MAPK Ras CaM kinetics[21] PKC PLA2 MAPK Ras CaM 
    kinetics[22] PKC PLA2 MAPK Ras CaM kinetics[23] PKC PLA2 
    MAPK Ras CaM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times.
    In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec.
    The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation.
    The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

    PLA2* acting as a Molecule in  
    Ajay_Bhalla_2007_ReacDiff3 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 920
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 922
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 933
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 939
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 945
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 951
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 957
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1000
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 964
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 970
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 976
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 982
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 988
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 994
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1005
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1011
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1017
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1023
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1029
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1035
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1041
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1047
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1053
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1059
    0125.7No
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1065
    0125.7No

    PLA2* acting as a Product of an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff3 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    2MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics
    Pathway No. : 926
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    3MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[1]
    Pathway No. : 931
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    4MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[2]
    Pathway No. : 937
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    5MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[3]
    Pathway No. : 943
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    6MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[4]
    Pathway No. : 949
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    7MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[5]
    Pathway No. : 955
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    8MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[6]
    Pathway No. : 962
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    9MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[7]
    Pathway No. : 968
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    10MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[8]
    Pathway No. : 975
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    11MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[9]
    Pathway No. : 980
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    12MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[10]
    Pathway No. : 986
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    13MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[11]
    Pathway No. : 992
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    14MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[12]
    Pathway No. : 998
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    15MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[13]
    Pathway No. : 1003
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    16MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[14]
    Pathway No. : 1009
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    17MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[15]
    Pathway No. : 1015
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    18MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[16]
    Pathway No. : 1020
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    19MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[17]
    Pathway No. : 1027
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    20MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[18]
    Pathway No. : 1033
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    21MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[19]
    Pathway No. : 1039
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    22MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[20]
    Pathway No. : 1045
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    23MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[21]
    Pathway No. : 1051
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    24MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[22]
    Pathway No. : 1057
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
    25MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[23]
    Pathway No. : 1063
    25.6405104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
        Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

    PLA2* acting as a Substrate in a reaction in  
    Ajay_Bhalla_2007_ReacDiff3 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    2PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 920
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    3
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 920
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    4diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    5PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 922
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    6
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 922
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    7diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    8PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 933
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    9
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 933
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    10diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    11PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 939
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    12
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 939
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    13diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    14PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 945
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    15
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 945
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    16diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    17PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 951
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    18
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 951
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    19diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    20PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 957
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    21
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 957
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    22diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    23PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1000
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    24
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1000
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    25diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    26PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 964
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    27
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 964
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    28diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    29PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 970
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    30
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 970
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    31diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    32PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 976
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    33
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 976
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    34diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    35PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 982
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    36
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 982
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    37diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    38PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 988
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    39
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 988
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    40diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    41PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 994
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    42
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 994
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    43diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    44PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1005
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    45
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1005
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    46diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    47PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1011
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    48
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1011
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    49diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    50PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1017
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    51
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1017
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    52diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    53PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1023
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    54
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1023
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    55diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    56PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1029
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    57
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1029
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    58diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    59PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1035
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    60
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1035
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    61diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    62PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1041
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    63
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1041
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    64diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    65PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1047
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    66
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1047
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    67diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    68PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1053
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    69
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1053
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    70diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    71PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1059
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    72
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1059
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic
    73PLA2*-Ca-act
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1065
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    74
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • PLA2
    Pathway No. : 1065
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic

    PLA2* acting as a Product in a reaction in  
    Ajay_Bhalla_2007_ReacDiff3 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    2diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    3diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    4diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    5diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    6diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    7diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    8diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    9diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    10diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    11diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    12diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    13diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    14diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    15diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    16diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    17diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    18diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    19diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    20diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    21diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    22diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    23diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*
    24diff
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 0.001
    (s^-1)
    0.001
    (s^-1)
    Keq = 1(uM)500secSubstrate
    PLA2*

    Product
    PLA2*



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