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Molecule Parameter List for Rec-Gq

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Rec-Gq participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000021

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Osc_Ca_
    IP3metabolism
  • 24Network
    MIPP CaMKII CaM 
    PKC IP3-3K Gq 
    PLCbeta 134_dephos 145_dephos 
    IP4-system IHP-system 1345_dephos 
    CaRegulation Othmer-Tang-model 
    This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    Rec-Gq acting as a Molecule in  
    Osc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    Rec-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • Gq
    Pathway No. : 125
    01000No
    Turns out that a large fraction of the the receptor binds to the G-protein even in the absence of ligand. This pool represents this step. Fraction of Rec-Gq is 44% of receptor, from Fay et al 1991 Biochem 30:5066-5075 Since this is not the same receptor, this value is a bit doubtful. Still, we adjust the rate consts in Rec-bind-Gq to match.

    Rec-Gq acting as a Substrate in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Glu-bind-Rec-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • Gq
    Pathway No. : 125
    16.8
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.006(uM)-Substrate
    Glu
    Rec-Gq

    Product
    Rec-Glu-Gq
      From Fay et al kb3 = kb = 1.06e-3 which is rather slow. k+1 = kf = 2.8e7 /M/sec= 4.67e-5/sec use 5e-5. However, the Kd from Martin et al may be more appropriate, as this is Glu not the system from Fay. kf = 2.8e-5, kb = 10 Let us compromise. since we have the Fay model, keep kf = k+1 = 2.8e-5. But kb (k-3) is .01 * k-1 from Fay. Scaling by .01, kb = .01 * 10 = 0.1
    2
  • Antag-bind-Rec-G
    q
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • Gq
    Pathway No. : 125
    60
    (uM^-1 s^-1)
    0.01
    (s^-1)
    Kd(bf) = 0.0002(uM)-Substrate
    Rec-Gq
    mGluRAntag

    Product
    Blocked-rec-Gq
      The rate consts give a total binding affinity of under 0.2 nM, good for a strong antagonist.

    Rec-Gq acting as a Product in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    Rec-bind-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • Gq
    Pathway No. : 125
    0.6
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 1.6667(uM)-Substrate
    G-GDP
    mGluR

    Product
    Rec-Gq
    From Berstein et al 1992 JBC 267(12):8081-8088 we know that 15-40% of Gq binds, GTP_gamma_S. Also about 20-30% of Gq is bound to GTP. To get to these values the receptor-Gq amount should be similar. These rates are designed to give that steady state with a fast tau of 1 sec.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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