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Molecule Parameter List for PLC

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLC participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010020

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Osc_Ca_
    IP3metabolism
  • 24Network
    MIPP CaMKII CaM 
    PKC IP3-3K Gq 
    PLCbeta 134_dephos 145_dephos 
    IP4-system IHP-system 1345_dephos 
    CaRegulation Othmer-Tang-model 
    This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    PLC acting as a Molecule in  
    Osc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLC
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • PLCbeta
    Pathway No. : 126
    0.041000No
    Smrcka et al; Science 251, 15.2.1991, pp804-807

    PLC acting as an Enzyme in  
    Osc_Ca_IP3metabolism Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PLC /
    PLC
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • PLCbeta
    Pathway No. : 126
    19.99942.54explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
    Smrcka et al; Science 251, 15.2.1991, pp804-807

    PLC acting as a Substrate in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Act-PLC-Ca
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • PLCbeta
    Pathway No. : 126
    3
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.3333(uM)-Substrate
    Ca
    PLC

    Product
    PLC-Ca
      Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 so [Ca].kf = kb so kb/kf = 1 * 6e5 = 1/1.66e-6 Assigned affinity to a Kd of 0.333 to maintain balance.
    2PLC-bind-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • PLCbeta
    Pathway No. : 126
    2.52
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.3968(uM)-Substrate
    G*GTP
    PLC

    Product
    PLC-Gq
      this binding does not produce active PLC. This step was needed to implement the described (Smrcka et al) increase in affinity for Ca by PLC once Gq was bound. The tempkin are the same as the binding step for Ca-PLC to Gq. Kd is constrained by detailed balance.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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