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Molecule Parameter List for PKA-active

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKA-active participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010012

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
cAMP_pathway25Network
Shared_Object_cAMP_pathway PKA AC 
Gs 
This is a model of the canonical cAMP signaling pathway:
Ligand->Receptor->G-protein->Cyclase->cAMP->PKA.
It also includes phosphodiesterases to balance out cAMP formation.Bhalla US Methods Enzymol. 2002;345:3-23

PKA-active acting as a Molecule in  
cAMP_pathway Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
PKA-activecAMP_pathway
Accession No. : 25
  • Shared_Object_
    cAMP_pathway

    Pathway No. : 134
  • 01000No

    PKA-active acting as an Enzyme in  
    cAMP_pathway Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PKA-active /
    phosph-PDE
    cAMP_pathway
    Accession No. : 25
  • Shared_Object_
    cAMP_pathway

    Pathway No. : 134
  • 7.594explicit E-S complexSubstrate
    cAMP-PDE

    Product
    cAMP-PDE*
    See Bramson et al CRC crit rev Biochem 15:2 93-124. The rates there are for peptide substrates and too fast. Scaled down by a factor of 3 as per Cohen et al FEBS Lett 76:182-86 (1977).

    PKA-active acting as a Substrate in a reaction in  
    cAMP_pathway Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    inhib-PKAcAMP_pathway
    Accession No. : 25
    PKA
    Pathway No. : 135
    60
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    PKA-active
    PKA-inhibitor

    Product
    inhibited-PKA
    This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though.

    PKA-active acting as a Product in a reaction in  
    cAMP_pathway Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Release-C1cAMP_pathway
    Accession No. : 25
    PKA
    Pathway No. : 135
    60
    (s^-1)
    18
    (uM^-1 s^-1)
    Kd(cb) = 0.3(uM)-Substrate
    cAMP4.R2C2

    Product
    PKA-active
    cAMP4.R2C
      This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10
    2Release-C2cAMP_pathway
    Accession No. : 25
    PKA
    Pathway No. : 135
    60
    (s^-1)
    18
    (uM^-1 s^-1)
    Kd(cb) = 0.3(uM)-Substrate
    cAMP4.R2C

    Product
    PKA-active
    cAMP4.R2



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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