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Molecule Parameter List for GTP.Ga

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
GTP.Ga participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000021

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
cAMP_pathway25Network
Shared_Object_cAMP_pathway PKA AC 
Gs 
This is a model of the canonical cAMP signaling pathway:
Ligand->Receptor->G-protein->Cyclase->cAMP->PKA.
It also includes phosphodiesterases to balance out cAMP formation.Bhalla US Methods Enzymol. 2002;345:3-23

GTP.Ga acting as a Molecule in  
cAMP_pathway Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
GTP.GacAMP_pathway
Accession No. : 25
  • Shared_Object_
    cAMP_pathway

    Pathway No. : 134
  • 01000No
    Activated G protein. Supposed to be 15-40% of total Gs when max stim.

    GTP.Ga acting as a Substrate in a reaction in  
    cAMP_pathway Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Gs-bind-ACcAMP_pathway
    Accession No. : 25
    AC
    Pathway No. : 136
    499.998
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.002(uM)-Substrate
    AC
    GTP.Ga

    Product
    Gs.AC
      Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer Jacobowitz thesis (Mount Sinai 1995) data indicates it is more like 2 nM.
    2GTPasecAMP_pathway
    Accession No. : 25
    Gs
    Pathway No. : 137
    0.0667
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP.Ga

    Product
    GDP.Ga
      From Brandt and Ross JBC 261(4):1656-1664, rate is 4/min and is agonist independent. I treat this as irreversible.

    GTP.Ga acting as a Product in a reaction in  
    cAMP_pathway Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    Activate-GscAMP_pathway
    Accession No. : 25
    Gs
    Pathway No. : 137
    0.025
    (s^-1)
    0
    (uM^-2 s^-1)
    --Substrate
    L.R.GDP.Gabc

    Product
    GTP.Ga
    Gbg
    L.R
    This step combines several stages in GTP.Galpha release. From Berstein et al activation is at .35 - 0.7/min From Fay et al Biochem 30 5066-5075 1991 kf = .01/sec. From Brandt and Ross JBC 261(4):1656-1664 (1986) and Ransan et al Biochem J 283(2):519-524 (1992) rates around 2.5/min to 1.5/min are better.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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