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Molecule Parameter List for AMP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
AMP participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000200

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
cAMP_pathway25Network
Shared_Object_cAMP_pathway PKA AC 
Gs 
This is a model of the canonical cAMP signaling pathway:
Ligand->Receptor->G-protein->Cyclase->cAMP->PKA.
It also includes phosphodiesterases to balance out cAMP formation.Bhalla US Methods Enzymol. 2002;345:3-23

AMP acting as a Molecule in  
cAMP_pathway Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
AMPcAMP_pathway
Accession No. : 25
AC
Pathway No. : 136
10001000Yes
Assumed buffered to 1 mM. Value is irrelevant to simulation.

AMP acting as a Product of an Enzyme in  
cAMP_pathway Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1cAMP-PDE  /
PDE
cAMP_pathway
Accession No. : 25
AC
Pathway No. : 136
19.8413104explicit E-S complexSubstrate
cAMP

Product
AMP
    Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
2cAMP-PDE*  /
PDE*
cAMP_pathway
Accession No. : 25
AC
Pathway No. : 136
19.8413204explicit E-S complexSubstrate
cAMP

Product
AMP
    This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We'll ignore cGMP effects for now.



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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