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Molecule Parameter List for PKC-Ca

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-Ca participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000031

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
PKC_turnover26Pathway
PKC 
This model is a superset of the PKC model presented in Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. The current version includes PKC turnover. This version includes the stimulating pools Ca, AA and DAG as well as the PKC activity within the synaptic signaling pathway since it is meant to be a self-contained pathway model rather than part of a network.

PKC-Ca acting as a Molecule in  
PKC_turnover Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
PKC-CaPKC_turnover
Accession No. : 26
PKC
Pathway No. : 138
01000No
This intermediate is strongly indicated by the synergistic activation of PKC by combinations of DAG and Ca, as well as AA and Ca. PKC by definition also has a direct Ca-activation, to which this also contributes.

PKC-Ca acting as a Substrate in a reaction in  
PKC_turnover Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1PKC-act-by-DAGPKC_turnover
Accession No. : 26
PKC
Pathway No. : 138
0.008
(uM^-1 s^-1)
8.6348
(s^-1)
Kd(bf) = 1079.377(uM)-Substrate
DAG
PKC-Ca

Product
PKC-Ca-DAG
  Ca.PKC interaction with DAG is modeled by this reaction. Kf based on Shinomura et al PNAS 88 5149-5153 1991 and Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and uses the constraining procedure referred to in the general notes for PKC.
2PKC-Ca-to-membPKC_turnover
Accession No. : 26
PKC
Pathway No. : 138
1.2705
(s^-1)
3.5026
(s^-1)
Keq = 2.7569(uM)0.21secSubstrate
PKC-Ca

Product
PKC-Ca-memb*
  Membrane translocation is a standard step in PKC activation. It also turns out to be necessary to replicate the curves from Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and Shonomura et al 1991 PNAS 88:5149-5153. These rates are constrained by matching the curves in the above papers and by fixing a rather fast (sub-second) tau for PKC activation.
3
  • PKC-act-by-Ca-AA
  • PKC_turnover
    Accession No. : 26
    PKC
    Pathway No. : 138
    0.0012
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 83.3333(uM)-Substrate
    AA
    PKC-Ca

    Product
    PKC-Ca-AA*
      Ca-dependent AA activation of PKC. Note that this step combines the AA activation and also the membrane translocation. From Schaechter and Benowitz 1993 J Neurosci 13(10):4361

    PKC-Ca acting as a Product in a reaction in  
    PKC_turnover Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    PKC-act-by-CaPKC_turnover
    Accession No. : 26
    PKC
    Pathway No. : 138
    0.6
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.8333(uM)-Substrate
    Ca
    PKC-cytosolic

    Product
    PKC-Ca
    This Kd is a straightforward result from the Schaechter and Benowitz 1993 J Neurosci 13(10):4361 curves. The time-course is based on the known rapid activation of PKC and also the fact that Ca association with proteins is typicmpdmyy quite fast. My guess is that this tau of 2 sec is quite conservative and the actualy rate may be much faster. The parameter is quite insensitive for most stimuli.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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