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Result: 1 - 7 of 7 rows are displayed

Reaction List for pathway PKA (Pathway Number 240) in Accession AMPAR_traff_model0 (Accession Number 59)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 
  • cAMP-bind-site-B
    1
  • PKA

    Pathway No. 240
    54
    (uM^-1 s^-1)
    33
    (s^-1)
    Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2
     cAMP

     Products:
     R2C2-cAMP
      Hasler et al FASEB J 6:2734-2741 1992 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al PNAS USA 78:3 1591-1595 1981 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell). Ogreid and Doskeland Febs Lett 129:2 287-292 1981 have figs suggesting time course of complete assoc is < 1 min.
    2 
  • cAMP-bind-site-B
    2
  • PKA

    Pathway No. 240
    54
    (uM^-1 s^-1)
    33
    (s^-1)
    Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2-cAMP
     cAMP

     Products:
     R2C2-cAMP2
      For now let us set this to the same Km (1e-7M) as site B. This gives kf/kb = .7e-7M * 1e6 / (6e5^2) : 1/(6e5^2) = 2e-13:2.77e-12 Smith et al have better values. They say that this is cooperative, so the consts are now kf/kb =8.3e-4
    3 
  • cAMP-bind-site-A
    1
  • PKA

    Pathway No. 240
    75.0006
    (uM^-1 s^-1)
    110
    (s^-1)
    Kd(bf) = 1.4667(uM)-  Substrate:
     R2C2-cAMP2
     cAMP

     Products:
     R2C2-cAMP3
    4 
  • cAMP-bind-site-A
    2
  • PKA

    Pathway No. 240
    75.0006
    (uM^-1 s^-1)
    32.5
    (s^-1)
    Kd(bf) = 0.4333(uM)-  Substrate:
     cAMP
     R2C2-cAMP3

     Products:
     R2C2-cAMP4
    5 Release-C1PKA

    Pathway No. 240
    60
    (s^-1)
    17.9998
    (uM^-1 s^-1)
    Kd(cb) = 0.3(uM)-  Substrate:
     R2C2-cAMP4

     Products:
     PKA-active
     R2C-cAMP4
      This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10
    6 Release-C2PKA

    Pathway No. 240
    60
    (s^-1)
    17.9998
    (uM^-1 s^-1)
    Kd(cb) = 0.3(uM)-  Substrate:
     R2C-cAMP4

     Products:
     PKA-active
     R2-cAMP4
    7 inhib-PKAPKA

    Pathway No. 240
    59.9994
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.0167(uM)-  Substrate:
     PKA-active
     PKA-inhibitor

     Products:
     inhibited-PKA
      This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though. kf = 1e-5 kb = 1

     
    Result: 1 - 7 of 7 rows are displayed



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