| Molecule Name/ Site Name | Km (uM) | kcat (1/s) | Ratio (k2/k3) | Enzyme Type | Substrate | Product |
1 |
Enzyme Activity: ip1_1pase
Enzyme Molecule: IP1_pase | 160.001 | 11.43 | 4 | explicit E-S complex | IP1(1)
| inositol
|
| from Gee et al, Biochem J 249; 1988 |
2 |
Enzyme Activity: ip1_4pase
Enzyme Molecule: IP1_pase | 190 | 39.33 | 4 | explicit E-S complex | IP1(4)
| inositol
|
| Ins(4)P1 4-phsophatase from Gee et al, BiochemJ 249; 1988 |
3 |
Enzyme Activity: ip2_1pase
Enzyme Molecule: IP_1pase | 5.00005 | 2 | 4 | explicit E-S complex | IP2(14)
| IP1(4)
|
| from Inhorn and Majerus, JBC 1987 Vmax reduced from 5.625 to 2 to maintain levels of IP2(14) |
4 |
Enzyme Activity: ip3_1pase
Enzyme Molecule: IP_1pase | 20.0021 | 4.125 | 4 | explicit E-S complex | IP3(134)
| IP2(34)
|
| Ins(134) 1-phosphatase activity of InsP 1-phosphatase from Inhorn and Majerus, JBC 262; 1987 |
5 |
Enzyme Activity: ip3_5pase1
Enzyme Molecule: IP_5pase1 | 3.00001 | 1.1 | 4 | explicit E-S complex | IP3(145)
| IP2(14)
|
| Ins(145)P3 5-phosphatase activity of InsP-5-phosphatase from Hansen et al, JBC 262; 1987 |
6 |
Enzyme Activity: ip3_5pase2
Enzyme Molecule: IP3_5pase2 | 18.0004 | 10.667 | 3.99991 | explicit E-S complex | IP3(145)
| IP2(14)
|
| from Hansen et al, JBC 262, 1987 activity determined from pH curve. Value scaled 1.5 times to obtain activity at 37C as original experiment was performed at 30C |
7 |
Enzyme Activity: ip4_5pase
Enzyme Molecule: IP_5pase1 | 0.80003 | 0.09 | 4 | explicit E-S complex | IP4(1345)
| IP3(134)
|
| Ins(1345)P4 5-phosphatase activity of InsP 5-phosphatase1 from Hansen et al, JBC 262; 1987 vmax for IP4 substrate is approx 0.1 times vmax for IP3 |